(2R,3S,2″R,3″R)-Manniflavanone protects proliferating skeletal muscle cells against oxidative stress and stimulates myotube formation

Suresh Acharya, Timo D. Stark, Seung Tack Oh, Songhee Jeon, Sok Cheon Pak, Mina Kim, Jinyoung Hur, Toshiaki Matsutomo, Thomas Hofmann, Rodney A. Hill, Onesmo B. Balemba

Research output: Contribution to journalArticle

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Abstract

We investigated the antioxidative properties of (2R,3S,2″R,3″R)-manniflavanone (MF) using in vitro assays and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and oxygen radical absorbance capacity assays demonstrated that MF is a powerful antioxidant. This finding was supported by diphenylpicrylhydrazine radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and myosin heavy chain. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and up-regulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with a higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging reactive oxygen species and regulating myogenic proteins factors.

Original languageEnglish
Pages (from-to)3636-3646
Number of pages11
JournalJournal of Agricultural and Food Chemistry
Volume65
Issue number18
DOIs
Publication statusPublished - 10 May 2017

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Oxidative stress
Skeletal Muscle Fibers
myocytes
Muscle Cells
Muscle
skeletal muscle
Lactic Acid
Skeletal Muscle
Oxidative Stress
oxidative stress
Scavenging
Cells
lactates
myricetin
Ascorbic Acid
Assays
Reactive Oxygen Species
Antioxidants
Garcinia
Myosin Heavy Chains

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Acharya, Suresh ; Stark, Timo D. ; Oh, Seung Tack ; Jeon, Songhee ; Pak, Sok Cheon ; Kim, Mina ; Hur, Jinyoung ; Matsutomo, Toshiaki ; Hofmann, Thomas ; Hill, Rodney A. ; Balemba, Onesmo B. / (2R,3S,2″R,3″R)-Manniflavanone protects proliferating skeletal muscle cells against oxidative stress and stimulates myotube formation. In: Journal of Agricultural and Food Chemistry. 2017 ; Vol. 65, No. 18. pp. 3636-3646.
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abstract = "We investigated the antioxidative properties of (2R,3S,2″R,3″R)-manniflavanone (MF) using in vitro assays and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and oxygen radical absorbance capacity assays demonstrated that MF is a powerful antioxidant. This finding was supported by diphenylpicrylhydrazine radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and myosin heavy chain. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and up-regulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with a higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging reactive oxygen species and regulating myogenic proteins factors.",
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(2R,3S,2″R,3″R)-Manniflavanone protects proliferating skeletal muscle cells against oxidative stress and stimulates myotube formation. / Acharya, Suresh; Stark, Timo D.; Oh, Seung Tack; Jeon, Songhee; Pak, Sok Cheon; Kim, Mina; Hur, Jinyoung; Matsutomo, Toshiaki; Hofmann, Thomas; Hill, Rodney A.; Balemba, Onesmo B.

In: Journal of Agricultural and Food Chemistry, Vol. 65, No. 18, 10.05.2017, p. 3636-3646.

Research output: Contribution to journalArticle

TY - JOUR

T1 - (2R,3S,2″R,3″R)-Manniflavanone protects proliferating skeletal muscle cells against oxidative stress and stimulates myotube formation

AU - Acharya, Suresh

AU - Stark, Timo D.

AU - Oh, Seung Tack

AU - Jeon, Songhee

AU - Pak, Sok Cheon

AU - Kim, Mina

AU - Hur, Jinyoung

AU - Matsutomo, Toshiaki

AU - Hofmann, Thomas

AU - Hill, Rodney A.

AU - Balemba, Onesmo B.

PY - 2017/5/10

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N2 - We investigated the antioxidative properties of (2R,3S,2″R,3″R)-manniflavanone (MF) using in vitro assays and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and oxygen radical absorbance capacity assays demonstrated that MF is a powerful antioxidant. This finding was supported by diphenylpicrylhydrazine radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and myosin heavy chain. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and up-regulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with a higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging reactive oxygen species and regulating myogenic proteins factors.

AB - We investigated the antioxidative properties of (2R,3S,2″R,3″R)-manniflavanone (MF) using in vitro assays and examined its effects on myogenesis and lactate-induced oxidative stress in C2C12 cells. MF was purified from Garcinia buchananii stem bark. H2O2 and oxygen radical absorbance capacity assays demonstrated that MF is a powerful antioxidant. This finding was supported by diphenylpicrylhydrazine radical scavenging activity of MF. MF was less cytotoxic to C2C12 cells compared to ascorbic acid and myricetin. Moreover, MF accelerated myotube formation in the differentiated C2C12 cells by up-regulating myogenic proteins such as MyoG and myosin heavy chain. Furthermore, MF rescued late differentiation of myoblast suppressed by lactate treatment and up-regulated the expression levels of Nrf2 in lactate-induced oxidative stress, indicating that MF stimulates antioxidative activity inside C2C12 cells. Collectively, MF is a potent antioxidant with a higher safety profile than ascorbic acid and myricetin. It reduces oxidative stress-induced delaying of skeletal muscle differentiation by scavenging reactive oxygen species and regulating myogenic proteins factors.

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