A general target selection method for crystallographic proteomics

Gautier Robin, Nathan P. Cowieson, Gregor Guncar, Jade Forwood, Pawel Listwan, David Hume, Bostjan Kobe, Jennifer L. Martin, Thomas Huber

Research output: Book chapter/Published conference paperChapter

1 Citation (Scopus)

Abstract

Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and 'crystallizability,' the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their 'crystallizability' estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.
Original languageEnglish
Title of host publicationStructural proteomics
Subtitle of host publicationhigh-throughput methods
Place of PublicationTotowa, N.J.
PublisherHumana Press
Pages27-35
Number of pages9
Volume426
Edition2
ISBN (Print)9781588298096
DOIs
Publication statusPublished - 2008

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    Robin, G., Cowieson, N. P., Guncar, G., Forwood, J., Listwan, P., Hume, D., Kobe, B., Martin, J. L., & Huber, T. (2008). A general target selection method for crystallographic proteomics. In Structural proteomics: high-throughput methods (2 ed., Vol. 426, pp. 27-35). Humana Press. https://doi.org/10.1007/978-1-60327-058-8_2