A general target selection method for crystallographic proteomics

Gautier Robin; Nathan P. Cowieson; Gregor Guncar; Jade Forwood; Pawel Listwan; David Hume; Bostjan Kobe; Jennifer L. Martin; Thomas Huber

doi:10.1007/978-1-60327-058-8_2

A general target selection method for crystallographic proteomics

Gautier Robin, Nathan P. Cowieson, Gregor Guncar, Jade Forwood, Pawel Listwan, David Hume, Bostjan Kobe, Jennifer L. Martin, Thomas Huber

Research output: Book chapter/Published conference paper › Chapter

1 Citation (Scopus)

Abstract

Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and 'crystallizability,' the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their 'crystallizability' estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.

Original language	English
Title of host publication	Structural proteomics
Subtitle of host publication	high-throughput methods
Place of Publication	Totowa, N.J.
Publisher	Humana Press
Pages	27-35
Number of pages	9
Volume	426
Edition	2
ISBN (Print)	9781588298096
DOIs	https://doi.org/10.1007/978-1-60327-058-8_2
Publication status	Published - 2008

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10.1007/978-1-60327-058-8_2

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title = "A general target selection method for crystallographic proteomics",

abstract = "Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and 'crystallizability,' the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their 'crystallizability' estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.",

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AU - Robin, Gautier

AU - Cowieson, Nathan P.

AU - Guncar, Gregor

AU - Forwood, Jade

AU - Listwan, Pawel

AU - Hume, David

AU - Kobe, Bostjan

AU - Martin, Jennifer L.

AU - Huber, Thomas

N1 - Imported on 12 May 2017 - DigiTool details were: publisher = Totowa, N.J: Humana Press, 2008. editor/s (773b) = Bostjan Hobe, Mitchell Guss, Thomas Huber ; Volume no. (773r) = 426; Issue no. (773s) = 2; Parent title (773t) = Structural proteomics: high-throughput methods; No. of chapters (773w) = 10.

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AB - Increasing the success in obtaining structures and maximizing the value of the structures determined are the two major goals of target selection in structural proteomics. This chapter presents an efficient and flexible target selection procedure supplemented with a Web-based resource that is suitable for small- to large-scale structural genomics projects that use crystallography as the major means of structure determination. Based on three criteria, biological significance, structural novelty, and 'crystallizability,' the approach first removes (filters) targets that do not meet minimal criteria and then ranks the remaining targets based on their 'crystallizability' estimates. This novel procedure was designed to maximize selection efficiency, and its prevailing criteria categories make it suitable for a broad range of structural proteomics projects.

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BT - Structural proteomics

PB - Humana Press

CY - Totowa, N.J.

ER -

A general target selection method for crystallographic proteomics

Abstract

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