A high throughput, non-organic method for plant genomic DNA isolation.

Ata-Ur Rahman, Ben Stodart, Harsh Raman

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

A simple and reliable method for extracting genomic DNA from leaf and seed of monocotyledonous and dicotyledonous plants and ascomycete fungal mycelium is described. The method requires only 50mg of sample and yields approximately 10µg of high quality DNA. It involves inexpensive, non-organic constituents and can reliably be used for the parallel isolation of 384 DNA samples suitable for PCR based marker analysis. The yield and high quality of extracted DNA from different species, combined with the use of inexpensive, non-hazardous reagents, provides a cost-effective method compatible with a 96 well format. The resultant DNA is suitable for PCR and subsequent fragment analysis using capillary electrophoresis.
Original languageEnglish
Pages (from-to)831-840
Number of pages10
JournalPakistan Journal of Botany
Volume39
Issue number3
Publication statusPublished - 2007

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