A method for discovery of genome-wide SNP between any two genotypes from whole-genome re-sequencing data

S. Gopala Krishnan, Daniel L.E. Waters, Robert J. Henry

    Research output: Book chapter/Published conference paperChapter in textbook/reference bookpeer-review

    1 Citation (Scopus)

    Abstract

    Advances in sequencing technologies have aided the discovery of millions of genome-wide DNA polymorphisms such as single-nucleotide polymorphisms (SNPs) and insertion-deletions (InDels) which are an invaluable resource for marker-assisted breeding. Presently available bioinformatics tools assist the discovery of polymorphisms between target genotypes and the reference genome for a range of species. The discovery of polymorphisms between two genotypes within a breeding program is complicated by several factors such as bias in the number of reads from each genotype and residual heterozygosity within each genotype. In this chapter, we describe a novel approach where polymorphisms between a pair of genotypes are discovered from whole-genome re-sequencing data.
    Original languageEnglish
    Title of host publicationCereal genomics
    Subtitle of host publicationMethods and protocols
    EditorsRobert J. Henry, Angelo Furtado
    Place of PublicationTotowa, NJ
    PublisherHumana Press Inc.
    Chapter24
    Pages287-294
    Number of pages8
    Volume1099
    ISBN (Electronic)9781627037150
    ISBN (Print)9781627037143
    DOIs
    Publication statusPublished - 2014

    Publication series

    NameMethods in Molecular Biology
    Volume1099
    ISSN (Print)1064-3745

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