A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity

Abu Ali Ibn Sina, Matthew Thomas Foster, Darren Korbie, Laura G. Carrascosa, Muhammad J.A. Shiddiky, Jing Gao, Shuvashis Dey, Matt Trau

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl-1 of DNA with no sequencing requirement.
Original languageEnglish
Pages (from-to)3573-3578
Number of pages6
JournalAnalyst
Volume142
Issue number19
Early online date16 Aug 2017
DOIs
Publication statusPublished - 07 Oct 2017

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