TY - JOUR
T1 - A multiplex microplatform for the detection of multiple DNA methylation events using gold-DNA affinity
AU - Sina, Abu Ali Ibn
AU - Foster, Matthew Thomas
AU - Korbie, Darren
AU - Carrascosa, Laura G.
AU - Shiddiky, Muhammad J.A.
AU - Gao, Jing
AU - Dey, Shuvashis
AU - Trau, Matt
N1 - Publisher Copyright:
© 2017 The Royal Society of Chemistry.
PY - 2017/10/7
Y1 - 2017/10/7
N2 - We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl-1 of DNA with no sequencing requirement.
AB - We report a new multiplexed strategy for the electrochemical detection of regional DNA methylation across multiple regions. Using the sequence dependent affinity of bisulfite treated DNA towards gold surfaces, the method integrates the high sensitivity of a micro-fabricated multiplex device comprising a microarray of gold electrodes, with the powerful multiplexing capability of multiplex-PCR. The synergy of this combination enables the monitoring of the methylation changes across several genomic regions simultaneously from as low as 500 pg μl-1 of DNA with no sequencing requirement.
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U2 - 10.1039/c7an00611j
DO - 10.1039/c7an00611j
M3 - Article
C2 - 28861578
AN - SCOPUS:85029911283
SN - 0003-2654
VL - 142
SP - 3573
EP - 3578
JO - Analyst
JF - Analyst
IS - 19
ER -