TY - JOUR
T1 - A multiplexed device based on tunable Nanoshearing for specific detection of multiple protein biomarkers in serum
AU - Vaidyanathan, Ramanathan
AU - Van Leeuwen, Lara Michelle
AU - Rauf, Sakandar
AU - Shiddiky, Muhammad J.A.
AU - Trau, Matt
N1 - Funding Information:
This work was supported by the ARC DECRA (DE120102503) and ARC DP (DP140104006). We also acknowledge funding received by our laboratory from the National Breast Cancer Foundation of Australia (CG-12-07). This grant has significantly contributed to the environment to stimulate the research described here. The fabrication work was performed at the Queensland node of the Australian National Fabrication Facility (ANFF).
Publisher Copyright:
© 2015 Macmillan Publishers Limited. All rights reserved.
PY - 2015/5/15
Y1 - 2015/5/15
N2 - Microfluidic flow based multiplexed devices have gained significant promise in detecting biomarkers in complex biological samples. However, to fully exploit their use in bioanalysis, issues such as (i) low sensitivity and (ii) high levels of nonspecific adsorption of non-target species have to be overcome. Herein, we describe a new multiplexed device for the sensitive detection of multiple protein biomarkers in serum by using an alternating current (ac) electrohydrodynamics (ac-EHD) induced surface shear forces based phenomenon referred to as nanoshearing. The tunable nature (via manipulation of ac field) of these nanoshearing forces can alter the capture performance of the device (e.g., improved fluid transport enhances number of sensor-target collisions). This can also selectively displace weakly (nonspecifically) bound molecules from the electrode surface (i.e., fluid shear forces can be tuned to shear away nonspecific species present in biological samples). Using this approach, we achieved sensitive (100 fg mL-1) naked eye detection of multiple protein targets spiked in human serum and a 1000-fold enhancement in comparison to hydrodynamic flow based devices for biomarker detection. We believe that this approach could potentially represent a clinical diagnostic tool that can be integrated into resource-limited settings for sensitive detection of target biomarkers using naked eye.
AB - Microfluidic flow based multiplexed devices have gained significant promise in detecting biomarkers in complex biological samples. However, to fully exploit their use in bioanalysis, issues such as (i) low sensitivity and (ii) high levels of nonspecific adsorption of non-target species have to be overcome. Herein, we describe a new multiplexed device for the sensitive detection of multiple protein biomarkers in serum by using an alternating current (ac) electrohydrodynamics (ac-EHD) induced surface shear forces based phenomenon referred to as nanoshearing. The tunable nature (via manipulation of ac field) of these nanoshearing forces can alter the capture performance of the device (e.g., improved fluid transport enhances number of sensor-target collisions). This can also selectively displace weakly (nonspecifically) bound molecules from the electrode surface (i.e., fluid shear forces can be tuned to shear away nonspecific species present in biological samples). Using this approach, we achieved sensitive (100 fg mL-1) naked eye detection of multiple protein targets spiked in human serum and a 1000-fold enhancement in comparison to hydrodynamic flow based devices for biomarker detection. We believe that this approach could potentially represent a clinical diagnostic tool that can be integrated into resource-limited settings for sensitive detection of target biomarkers using naked eye.
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U2 - 10.1038/srep09756
DO - 10.1038/srep09756
M3 - Article
C2 - 25978807
AN - SCOPUS:84929376617
SN - 2045-2322
VL - 5
JO - Scientific Reports
JF - Scientific Reports
M1 - 9756
ER -