Abstract
Nematodes have been thought to convert dietary cholesterol to 8(14)-lophenol by a secondary metabolism pathway that might involve sterol C4 methylation analogous to C24 methylation of sterols or C2 methylation of hopanoids. We characterized dichotomous cholesterol metabolic pathways in Caenorhabditis elegans that produce 3-oxo sterol intermediates in different paths en route to lophanol and 8(14)-lophenol. We detected alternate C3-sterol oxidation and desaturation steps that regulate sterol flux, while lophanol/8(14)-lophenol production was shown to be dependent on a sterol C4α-methyltransferse (4-SMT). 4-SMT catalyzes a 3-keto-enol tautomerism mechanism linked to SAM-dependent methylation and contributes to sterol diversity.
| Original language | English |
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| Publication status | Published - 2022 |
| Event | ComBio2022 - Melbourne Convention and Exhibition Centre, Melbourne , Australia Duration: 28 Sept 2022 → 30 Sept 2022 https://www.asps.org.au/conferences/combio2022 (Conference website) |
Conference
| Conference | ComBio2022 |
|---|---|
| Country/Territory | Australia |
| City | Melbourne |
| Period | 28/09/22 → 30/09/22 |
| Other | Due to the COVID-19 pandemic Combio2020 was postponed. The conference will now be called “ComBio2022” and will still be held at the Melbourne Convention and Exhibition Centre but from Tuesday, 27 September 2022 (registration in late afternoon), with the full program commencing on Wednesday, 28 September and running through to Friday, 30 September 2022 inclusive. Thanks to our great organizing committee, the program is already very advanced with most of the symposium sessions, chairs and invited speakers in place. Program and Registration details: ComBio2022 |
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