Nematodes have been thought to convert dietary cholesterol to 8(14)-lophenol by a secondary metabolism pathway that might involve sterol C4 methylation analogous to C24 methylation of sterols or C2 methylation of hopanoids. We characterized dichotomous cholesterol metabolic pathways in Caenorhabditis elegans that produce 3-oxo sterol intermediates in different paths en route to lophanol and 8(14)-lophenol. We detected alternate C3-sterol oxidation and desaturation steps that regulate sterol flux, while lophanol/8(14)-lophenol production was shown to be dependent on a sterol C4α-methyltransferse (4-SMT). 4-SMT catalyzes a 3-keto-enol tautomerism mechanism linked to SAM-dependent methylation and contributes to sterol diversity.
|Publication status||Published - 2022|
|Event||ComBio2022 - Melbourne Convention and Exhibition Centre, Melbourne , Australia|
Duration: 28 Sep 2022 → 30 Sep 2022
|Period||28/09/22 → 30/09/22|