A nematode sterol Cα4-methyltransferase (4-SMT) contributes to sterol diversity

Wenxu Zhou, Paxtyn M. Fisher, Boden H. Vanderloop, Yun Shen, Huazhong Shi, Adrian J. Maldonado, David Leaver, W. David Nes

Research output: Other contribution to conferencePosterpeer-review


Nematodes have been thought to convert dietary cholesterol to 8(14)-lophenol by a secondary metabolism pathway that might involve sterol C4 methylation analogous to C24 methylation of sterols or C2 methylation of hopanoids. We characterized dichotomous cholesterol metabolic pathways in Caenorhabditis elegans that produce 3-oxo sterol intermediates in different paths en route to lophanol and 8(14)-lophenol. We detected alternate C3-sterol oxidation and desaturation steps that regulate sterol flux, while lophanol/8(14)-lophenol production was shown to be dependent on a sterol C4α-methyltransferse (4-SMT). 4-SMT catalyzes a 3-keto-enol tautomerism mechanism linked to SAM-dependent methylation and contributes to sterol diversity.
Original languageEnglish
Publication statusPublished - 2022
EventComBio2022 - Melbourne Convention and Exhibition Centre, Melbourne , Australia
Duration: 28 Sept 202230 Sept 2022
https://www.asps.org.au/conferences/combio2022 (Conference website)


OtherDue to the COVID-19 pandemic Combio2020 was postponed. The conference will now be called “ComBio2022” and will still be held at the Melbourne Convention and Exhibition Centre but from Tuesday, 27 September 2022 (registration in late afternoon), with the full program commencing on Wednesday, 28 September and running through to Friday, 30 September 2022 inclusive. Thanks to our great organizing committee, the program is already very advanced with most of the symposium sessions, chairs and invited speakers in place. Program and Registration details: ComBio2022
Internet address


Dive into the research topics of 'A nematode sterol Cα4-methyltransferase (4-SMT) contributes to sterol diversity'. Together they form a unique fingerprint.

Cite this