A nematode sterol Cα4-methyltransferase (4-SMT) contributes to sterol diversity

Wenxu Zhou, Paxtyn M. Fisher, Boden H. Vanderloop, Yun Shen, Huazhong Shi, Adrian J. Maldonado, David Leaver, W. David Nes

Research output: Other contribution to conferencePosterpeer-review


Nematodes have been thought to convert dietary cholesterol to 8(14)-lophenol by a secondary metabolism pathway that might involve sterol C4 methylation analogous to C24 methylation of sterols or C2 methylation of hopanoids. We characterized dichotomous cholesterol metabolic pathways in Caenorhabditis elegans that produce 3-oxo sterol intermediates in different paths en route to lophanol and 8(14)-lophenol. We detected alternate C3-sterol oxidation and desaturation steps that regulate sterol flux, while lophanol/8(14)-lophenol production was shown to be dependent on a sterol C4α-methyltransferse (4-SMT). 4-SMT catalyzes a 3-keto-enol tautomerism mechanism linked to SAM-dependent methylation and contributes to sterol diversity.
Original languageEnglish
Publication statusPublished - 2022
EventComBio2022 - Melbourne Convention and Exhibition Centre, Melbourne , Australia
Duration: 28 Sep 202230 Sep 2022


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