A new method for determination of herbicide resistance: Using Diversity Array Technology to determine annual ryegrass resistance to Trifluralin (Group D herbicide)

Aaron Preston, James Pratley, Joseph Moore, Andrzej Kilian

Research output: Book chapter/Published conference paperConference paperpeer-review

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Abstract

Annual ryegrass (Lolium rigidum Gaud.) is considered the worst winter crop weed of Australia. This is due in part to its propensity to evolve resistance to herbicides, having evolved resistance to 11 different herbicide groups. As levels of herbicide resistance in Australia are increasing, new methods are needed to assist in the early identification and treatment of resistant weeds. Conventional methods for the determination of resistance involve glasshouse pot trials, Petri dish assays or enzymatic determination, all of which consume considerable time and resources. A pilot study was conducted to determine the feasibility of utilising diversity arrays technology (DArT) as a faster and cheaper method for herbicide resistance testing. DArT is a high throughput genotyping technology which utilises microarrays for the discovery of genetic markers. The aim was to identify genetic markers in resistant and susceptible biotypes of annual ryegrass and to assess the ability of DArT to discriminate between herbicide resistant phenotypes.
Original languageEnglish
Title of host publicationProceedings of the 19th Australasian Weed Conference (2014)
Subtitle of host publicationScience, Community and Food Security: the Weed Challenge
EditorsMatthew Baker
Place of PublicationHobart
PublisherTasmanian Weed Society Inc
Pages210-213
Number of pages4
ISBN (Electronic)978-0-646-92454-0
Publication statusPublished - 2014
Event19th Australasian Weeds Conference (2014) - Hotel Grand Chancellor, Hobart, Australia
Duration: 01 Sep 201404 Sep 2014
http://caws.org.au/awc_contents.php?yr=2014 (Conference proceedings)
http://caws.org.nz/old-site/

Conference

Conference19th Australasian Weeds Conference (2014)
Abbreviated titleScience, Community and Food Security: the Weed Challenge
Country/TerritoryAustralia
CityHobart
Period01/09/1404/09/14
Internet address

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