A novel ultrasensitive phosphate amperometric nanobiosensor based on the integration of pyruvate oxidase with highly ordered gold nanowires array

Edward Ogabiela, Samuel B. Adeloju, Jiewu Cui, Yucheng Wu, Wei Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A novel phosphate amperometric nanobiosensor, based on an intimate integration of pyruvate oxidase (PyOx) and its cofactors, thiamine pyrophosphate (TPP) and flavin adenine dinucleotide (FAD), with a highly ordered gold nanowires array (AuNWA) has been developed. The successful integration of PyOx and the co-factors, via crosslinking with bovine serum albumin (BSA) and glutaraldehyde (GLA), onto the AuNWA was confirmed by cyclic voltammetry and amperometry. The resulting nanobiosensor achieved a detection limit of 0.1μM, a linear concentration range of 12.5-1000μM, and a sensitivity of 140.3μAmM-1cm-2. Notably, the incorporation of the AuNWA reduced the required PyOx concentration by 70-120 fold and the presence of common interferants, such as chloride, sulfate, fluoride, nitrite and nitrate ions did not interfere with phosphate detection. Furthermore, the nanobiosensor demonstrated a very high stability with repeated use over two weeks and was successfully used for the determination of phosphate in water samples with an average recovery of 96.6±4.9%.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalBiosensors and Bioelectronics
Volume71
Early online date14 Apr 2015
DOIs
Publication statusPublished - 15 Sep 2015

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