A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

Gayle Petersen, Bryan Hilbert, Gareth Trope, Wouter Kalle, Padraig Strappe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Objectives: We investigated the applicability of single layer paper-based scaffolds for the three-dimensional (3D) growth and osteogenic differentiation of equine adipose-derived stem cells (EADSC), with comparison against conventional two-dimensional (2D) culture on polystyrene tissue culture vessels. Results: Viable culture of EADSC was achieved using paper-based scaffolds, with EADSC grown and differentiated in 3D culture retaining high cell viability (>94 %), similarly to EADSC in 2D culture. Osteogenic differentiation of EADSC was significantly enhanced in 3D culture, with Alizarin Red S staining and quantification demonstrating increased mineralisation (p < 0.0001), and an associated increase in expression of the osteogenic-specific markers alkaline phosphatase (p < 0.0001), osteopontin (p < 0.0001), and runx2 (p < 0.01). Furthermore, scanning electron microscopy revealed a spherical morphology of EADSC in 3D culture, compared to a flat morphology of EADSC in 2D culture. Conclusions: Single layer paper-based scaffolds provide an enhanced environment for the in vitro 3D growth and osteogenic differentiation of EADSC, with high cell viability, and a spherical morphology.
Original languageEnglish
Pages (from-to)2321-2331
Number of pages11
JournalBiotechnology Letters
Volume37
Issue number11
DOIs
Publication statusPublished - Nov 2015

Fingerprint

Horses
Stem Cells
Cell Survival
Osteopontin
Polystyrenes
Growth
Electron Scanning Microscopy
Alkaline Phosphatase
Staining and Labeling

Cite this

@article{9093fbf4fe9b4a229648570006d3b659,
title = "A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells",
abstract = "Objectives: We investigated the applicability of single layer paper-based scaffolds for the three-dimensional (3D) growth and osteogenic differentiation of equine adipose-derived stem cells (EADSC), with comparison against conventional two-dimensional (2D) culture on polystyrene tissue culture vessels. Results: Viable culture of EADSC was achieved using paper-based scaffolds, with EADSC grown and differentiated in 3D culture retaining high cell viability (>94 {\%}), similarly to EADSC in 2D culture. Osteogenic differentiation of EADSC was significantly enhanced in 3D culture, with Alizarin Red S staining and quantification demonstrating increased mineralisation (p < 0.0001), and an associated increase in expression of the osteogenic-specific markers alkaline phosphatase (p < 0.0001), osteopontin (p < 0.0001), and runx2 (p < 0.01). Furthermore, scanning electron microscopy revealed a spherical morphology of EADSC in 3D culture, compared to a flat morphology of EADSC in 2D culture. Conclusions: Single layer paper-based scaffolds provide an enhanced environment for the in vitro 3D growth and osteogenic differentiation of EADSC, with high cell viability, and a spherical morphology.",
keywords = "Equine adipose-derived stem cells, Osteogenic differentiation, Paper-based scaffold, Three-dimensional culture, Two-dimensional culture",
author = "Gayle Petersen and Bryan Hilbert and Gareth Trope and Wouter Kalle and Padraig Strappe",
note = "Includes bibliographical references.",
year = "2015",
month = "11",
doi = "10.1007/s10529-015-1898-x",
language = "English",
volume = "37",
pages = "2321--2331",
journal = "Biotechnology Techniques",
issn = "0141-5492",
publisher = "Springer Netherlands",
number = "11",

}

A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells. / Petersen, Gayle; Hilbert, Bryan; Trope, Gareth; Kalle, Wouter; Strappe, Padraig.

In: Biotechnology Letters, Vol. 37, No. 11, 11.2015, p. 2321-2331.

Research output: Contribution to journalArticle

TY - JOUR

T1 - A paper-based scaffold for enhanced osteogenic differentiation of equine adipose-derived stem cells

AU - Petersen, Gayle

AU - Hilbert, Bryan

AU - Trope, Gareth

AU - Kalle, Wouter

AU - Strappe, Padraig

N1 - Includes bibliographical references.

PY - 2015/11

Y1 - 2015/11

N2 - Objectives: We investigated the applicability of single layer paper-based scaffolds for the three-dimensional (3D) growth and osteogenic differentiation of equine adipose-derived stem cells (EADSC), with comparison against conventional two-dimensional (2D) culture on polystyrene tissue culture vessels. Results: Viable culture of EADSC was achieved using paper-based scaffolds, with EADSC grown and differentiated in 3D culture retaining high cell viability (>94 %), similarly to EADSC in 2D culture. Osteogenic differentiation of EADSC was significantly enhanced in 3D culture, with Alizarin Red S staining and quantification demonstrating increased mineralisation (p < 0.0001), and an associated increase in expression of the osteogenic-specific markers alkaline phosphatase (p < 0.0001), osteopontin (p < 0.0001), and runx2 (p < 0.01). Furthermore, scanning electron microscopy revealed a spherical morphology of EADSC in 3D culture, compared to a flat morphology of EADSC in 2D culture. Conclusions: Single layer paper-based scaffolds provide an enhanced environment for the in vitro 3D growth and osteogenic differentiation of EADSC, with high cell viability, and a spherical morphology.

AB - Objectives: We investigated the applicability of single layer paper-based scaffolds for the three-dimensional (3D) growth and osteogenic differentiation of equine adipose-derived stem cells (EADSC), with comparison against conventional two-dimensional (2D) culture on polystyrene tissue culture vessels. Results: Viable culture of EADSC was achieved using paper-based scaffolds, with EADSC grown and differentiated in 3D culture retaining high cell viability (>94 %), similarly to EADSC in 2D culture. Osteogenic differentiation of EADSC was significantly enhanced in 3D culture, with Alizarin Red S staining and quantification demonstrating increased mineralisation (p < 0.0001), and an associated increase in expression of the osteogenic-specific markers alkaline phosphatase (p < 0.0001), osteopontin (p < 0.0001), and runx2 (p < 0.01). Furthermore, scanning electron microscopy revealed a spherical morphology of EADSC in 3D culture, compared to a flat morphology of EADSC in 2D culture. Conclusions: Single layer paper-based scaffolds provide an enhanced environment for the in vitro 3D growth and osteogenic differentiation of EADSC, with high cell viability, and a spherical morphology.

KW - Equine adipose-derived stem cells

KW - Osteogenic differentiation

KW - Paper-based scaffold

KW - Three-dimensional culture

KW - Two-dimensional culture

U2 - 10.1007/s10529-015-1898-x

DO - 10.1007/s10529-015-1898-x

M3 - Article

C2 - 26140902

VL - 37

SP - 2321

EP - 2331

JO - Biotechnology Techniques

JF - Biotechnology Techniques

SN - 0141-5492

IS - 11

ER -