A parallel comparison of antigen candidates for development of an optimized serological diagnosis of Schistosomiasis japonica in the Philippines

Pengfei Cai, Kosala G. Weerakoon, Yi Mu, David U. Olveda, Xianyu Piao, Shuai Liu, Remigio M. Olveda, Qijun Chen, Allen G. Ross, Donald P. McManus

    Research output: Contribution to journalArticlepeer-review

    29 Citations (Scopus)

    Abstract

    Schistosoma japonicum is stubbornly persistent in China and the Philippines. Fast and accurate diagnostic tools are required to monitor effective control measures against schistosomiasis japonica. Promising antigen candidates for the serological diagnosis of schistosomiasis japonica have generally been identified from the Chinese strain of S. japonicum. However, the Chinese (SjC) and Philippine (SjP) strains of S. japonicum express a number of clear phenotypic differences, including aspects of host immune responses. This feature thereby emphasized the requirement to determine whether antigens identified as having diagnostic value for SjC infection are also suitable for the diagnosis of SjP infection. In the current study, 10 antigens were selected for comparison of diagnostic performance of the SjP infection using ELISA. On testing of sera from 180 subjects in the Philippines, SjSAP4 exhibited the best diagnostic performance with 94.03% sensitivity and 98.33% specificity using an optimized serum dilution. In another large scale testing with 412 serum samples, a combination (SjSAP4 + Sj23-LHD (large hydrophilic domain)) provided the best diagnostic outcome with 87.04% sensitivity and 96.67% specificity. This combination could be used in future for serological diagnosis of schistosomiasis in the Philippines, thereby representing an important component for monitoring integrated control measures.

    Original languageEnglish
    Pages (from-to)237-246
    Number of pages10
    JournalEBioMedicine
    Volume24
    Early online date18 Sep 2017
    DOIs
    Publication statusPublished - Oct 2017

    Fingerprint

    Dive into the research topics of 'A parallel comparison of antigen candidates for development of an optimized serological diagnosis of Schistosomiasis japonica in the Philippines'. Together they form a unique fingerprint.

    Cite this