A portable device for LAMP based detection of SARS-COV-2

Kamalalayam Rajan Sreejith; Muhammad Umer; Larissa Dirr; Benjamin Bailly; Patrice Guillon; Mark von Itzstein; Narshone Soda; Surasak Kasetsirikul; Muhammad J. A. Shiddiky; Nam Trung Nguyen

doi:10.3390/mi12101151

A portable device for LAMP based detection of SARS-COV-2

Kamalalayam Rajan Sreejith, Muhammad Umer, Larissa Dirr, Benjamin Bailly, Patrice Guillon, Mark von Itzstein, Narshone Soda, Surasak Kasetsirikul, Muhammad J. A. Shiddiky, Nam Trung Nguyen

Griffith University

Research output: Contribution to journal › Article › peer-review

16 Citations (Scopus)

28 Downloads (Pure)

Abstract

This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 10² starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

Original language	English
Article number	1151
Number of pages	9
Journal	Micromachines
Volume	12
Issue number	10
Early online date	24 Sept 2021
DOIs	https://doi.org/10.3390/mi12101151
Publication status	Published - Oct 2021

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348154053 Published articleFinal published version, 1.54 MBLicence: CC BY

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title = "A portable device for LAMP based detection of SARS-COV-2",

abstract = "This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.",

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AU - Guillon, Patrice

AU - von Itzstein, Mark

AU - Soda, Narshone

AU - Kasetsirikul, Surasak

AU - Shiddiky, Muhammad J. A.

AU - Nguyen, Nam Trung

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N2 - This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

AB - This paper reports the design, development, and testing of a novel, yet simple and low-cost portable device for the rapid detection of SARS-CoV-2. The device performs loop mediated isothermal amplification (LAMP) and provides visually distinguishable images of the fluorescence emitted from the samples. The device utilises an aluminium block embedded with a cartridge heater for isothermal heating of the sample and a single-board computer and camera for fluorescence detection. The device demonstrates promising results within 20 min using clinically relevant starting concentrations of the synthetic template. Time-to-signal data for this device are considerably lower compared to standard quantitative Polymerase Chain Reaction(qPCR) machine (~10–20 min vs. >38 min) for 1 × 102 starting template copy number. The device in its fully optimized and characterized state can potentially be used as simple to operate, rapid, sensitive, and inexpensive platform for population screening as well as point-of-need severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) detection and patient management.

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A portable device for LAMP based detection of SARS-COV-2

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