A potentiometric phosphate biosensor based on entrapment of pyruvate oxidase in a polypyrrole film

Edward Ogabiela, Samuel B. Adeloju

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

A polypyrrole based potentiometric phosphate biosensor has been fabricated by entrapment of pyruvate oxidase in a polypyrrole film. The optimum conditions for the fabrication of biosensors were 0.3 M pyrrole, a polymerization time of 120 s, an applied current density of 0.05 mA cm-2 and 2 U mL -1 of pyruvate oxidase. Under these conditions, the polypyrrole-pyruvate oxidase (PPy-PyOx) biosensor enabled the achievement of a minimum detectable concentration of 3 μM phosphate, a linear concentration range of 15-400 μM (r2 = 0.980). The biosensor was successfully applied to the detection of phosphate in lake water samples with excellent recoveries ranging from 99-100%.
Original languageEnglish
Pages (from-to)5290-5297
Number of pages8
JournalAnalytical Methods
Volume6
Issue number14
Early online date07 Feb 2014
DOIs
Publication statusPublished - 21 Jul 2014

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