A polypyrrole based potentiometric phosphate biosensor has been fabricated by entrapment of pyruvate oxidase in a polypyrrole film. The optimum conditions for the fabrication of biosensors were 0.3 M pyrrole, a polymerization time of 120 s, an applied current density of 0.05 mA cm-2 and 2 U mL -1 of pyruvate oxidase. Under these conditions, the polypyrrole-pyruvate oxidase (PPy-PyOx) biosensor enabled the achievement of a minimum detectable concentration of 3 μM phosphate, a linear concentration range of 15-400 μM (r2 = 0.980). The biosensor was successfully applied to the detection of phosphate in lake water samples with excellent recoveries ranging from 99-100%.
Ogabiela, E., & Adeloju, S. B. (2014). A potentiometric phosphate biosensor based on entrapment of pyruvate oxidase in a polypyrrole film. Analytical Methods, 6(14), 5290-5297. https://doi.org/10.1039/c3ay42172d