A single step electrochemical integration of gold nanoparticles, cholesterol oxidase, cholesterol esterase and mediator with polypyrrole films for fabrication of free and total cholesterol nanobiosensors

Mohd Zulkhairi Abdul Rahim, Gwen Govender-Hondros, Samuel B. Adeloju

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2 Citations (Scopus)

Abstract

The development of free and total cholesterol nanobiosensors based on a single step electrochemical integration of gold nanoparticles (AuNPs), cholesterol oxidase (COx), cholesterol esterase (CE) and a mediator with polypyrrole (PPy) films is described. The incorporation of the various components in the PPy films was confirmed by chronopotentiometry, cyclic voltammetry (CV), scanning electron microscopy, energy dispersive X-ray analysis (SEM-EDX), and Fourier transformed infrared (FTIR) spectroscopy. The free cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx, nanobiosensor achieved a minimum detectable concentration of 5 μM, a linear concentration range of 5–25 μM and a sensitivity of 1.6 µA cm−2 µM−1 in 0.05 M phosphate buffer (pH 7.00). For the total cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE, nanobiosensor which also involved the co-incorporation of cholesterol esterase (CE) with the other components, the achieved performances include a minimum detectable total cholesterol concentration of 25 μM, a broader linear concentration range of 25–170 μM and a lower sensitivity of 0.1 µA µM−1 cm−2. Owing to its high selectivity, the presence of common interferants did not affect the total cholesterol measurement with the PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE nanobiosensor. Both nanobiosensors were successfully used for direct and indirect determination of total cholesterol in human blood serum samples.

Original languageEnglish
Pages (from-to)418-428
Number of pages11
JournalTalanta
Volume189
Early online date12 Jun 2018
DOIs
Publication statusPublished - 01 Nov 2018

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Cholesterol Oxidase
Sterol Esterase
Gold
Nanoparticles
Cholesterol
Fabrication
Energy dispersive X ray analysis
Fourier Analysis
Electron Scanning Microscopy
Cyclic voltammetry
polypyrrole
Infrared spectroscopy
Spectrum Analysis
Buffers
Blood
Phosphates
X-Rays
Scanning electron microscopy
Serum

Cite this

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title = "A single step electrochemical integration of gold nanoparticles, cholesterol oxidase, cholesterol esterase and mediator with polypyrrole films for fabrication of free and total cholesterol nanobiosensors",
abstract = "The development of free and total cholesterol nanobiosensors based on a single step electrochemical integration of gold nanoparticles (AuNPs), cholesterol oxidase (COx), cholesterol esterase (CE) and a mediator with polypyrrole (PPy) films is described. The incorporation of the various components in the PPy films was confirmed by chronopotentiometry, cyclic voltammetry (CV), scanning electron microscopy, energy dispersive X-ray analysis (SEM-EDX), and Fourier transformed infrared (FTIR) spectroscopy. The free cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx, nanobiosensor achieved a minimum detectable concentration of 5 μM, a linear concentration range of 5–25 μM and a sensitivity of 1.6 µA cm−2 µM−1 in 0.05 M phosphate buffer (pH 7.00). For the total cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE, nanobiosensor which also involved the co-incorporation of cholesterol esterase (CE) with the other components, the achieved performances include a minimum detectable total cholesterol concentration of 25 μM, a broader linear concentration range of 25–170 μM and a lower sensitivity of 0.1 µA µM−1 cm−2. Owing to its high selectivity, the presence of common interferants did not affect the total cholesterol measurement with the PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE nanobiosensor. Both nanobiosensors were successfully used for direct and indirect determination of total cholesterol in human blood serum samples.",
keywords = "Cholesterol, Gold nanoparticles, Human blood serum, Mediator, Nanobiosensor, Polypyrrole",
author = "Rahim, {Mohd Zulkhairi Abdul} and Gwen Govender-Hondros and Adeloju, {Samuel B.}",
year = "2018",
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T1 - A single step electrochemical integration of gold nanoparticles, cholesterol oxidase, cholesterol esterase and mediator with polypyrrole films for fabrication of free and total cholesterol nanobiosensors

AU - Rahim, Mohd Zulkhairi Abdul

AU - Govender-Hondros, Gwen

AU - Adeloju, Samuel B.

PY - 2018/11/1

Y1 - 2018/11/1

N2 - The development of free and total cholesterol nanobiosensors based on a single step electrochemical integration of gold nanoparticles (AuNPs), cholesterol oxidase (COx), cholesterol esterase (CE) and a mediator with polypyrrole (PPy) films is described. The incorporation of the various components in the PPy films was confirmed by chronopotentiometry, cyclic voltammetry (CV), scanning electron microscopy, energy dispersive X-ray analysis (SEM-EDX), and Fourier transformed infrared (FTIR) spectroscopy. The free cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx, nanobiosensor achieved a minimum detectable concentration of 5 μM, a linear concentration range of 5–25 μM and a sensitivity of 1.6 µA cm−2 µM−1 in 0.05 M phosphate buffer (pH 7.00). For the total cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE, nanobiosensor which also involved the co-incorporation of cholesterol esterase (CE) with the other components, the achieved performances include a minimum detectable total cholesterol concentration of 25 μM, a broader linear concentration range of 25–170 μM and a lower sensitivity of 0.1 µA µM−1 cm−2. Owing to its high selectivity, the presence of common interferants did not affect the total cholesterol measurement with the PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE nanobiosensor. Both nanobiosensors were successfully used for direct and indirect determination of total cholesterol in human blood serum samples.

AB - The development of free and total cholesterol nanobiosensors based on a single step electrochemical integration of gold nanoparticles (AuNPs), cholesterol oxidase (COx), cholesterol esterase (CE) and a mediator with polypyrrole (PPy) films is described. The incorporation of the various components in the PPy films was confirmed by chronopotentiometry, cyclic voltammetry (CV), scanning electron microscopy, energy dispersive X-ray analysis (SEM-EDX), and Fourier transformed infrared (FTIR) spectroscopy. The free cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx, nanobiosensor achieved a minimum detectable concentration of 5 μM, a linear concentration range of 5–25 μM and a sensitivity of 1.6 µA cm−2 µM−1 in 0.05 M phosphate buffer (pH 7.00). For the total cholesterol, PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE, nanobiosensor which also involved the co-incorporation of cholesterol esterase (CE) with the other components, the achieved performances include a minimum detectable total cholesterol concentration of 25 μM, a broader linear concentration range of 25–170 μM and a lower sensitivity of 0.1 µA µM−1 cm−2. Owing to its high selectivity, the presence of common interferants did not affect the total cholesterol measurement with the PPy-NO3 --Fe(CN)6 4--AuNPs-COx-CE nanobiosensor. Both nanobiosensors were successfully used for direct and indirect determination of total cholesterol in human blood serum samples.

KW - Cholesterol

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KW - Human blood serum

KW - Mediator

KW - Nanobiosensor

KW - Polypyrrole

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