Casein and whey protein fractions of milk obtained from 47 ewes of five breeds or crossbreeds (Awassi, Merino, East FriesianÃ—Merino, AwassiÃ—Merino and AwassiÃ—East Friesian) were analysed by capillary electrophoresis (CE). The experiments were performed on a Beckman P/ACE' system 5510 with an uncoated fused-silica capillary and a low pH buffer containing urea and a polymeric additive. The four major caseins ('s1-, 's2-, ÃŸ- and '-casein) in an acid precipitate were well separated, as were the two whey proteins, '-lactalbumin ('-La) and ÃŸ-lactoglobulin (ÃŸ-Lg). The electromigration of the proteins was in the order of '-La, ÃŸ-Lg, 's2-CN, 's1-CN, '-CN and ÃŸ-CN. The milk samples were composed of the same variant of '-La and two different genotypes (A and B) of ÃŸ-Lg while the ÃŸ-Lg AB genotype was evident in the milk of some animals. The 's1-CN fractions displayed considerable heterogeneity with at least 4 different peaks, representing 4 different variants. A fifth peak, corresponding to the Welsh variant (or 's1-CN D), was present in 90% of the ewes' milk samples. The '-CN fraction was resolved as a single peak, while the ÃŸ-CN revealed significant heterogeneity with 3 variants. It appears that the presence of the 's1-CN Welsh variant in Merino ewe and its crosses with Awassi and East Friesian ewes adversely affected milk composition and yield.