Abrogation of the interferon response promotes more efficient human cytomegalovirus replication

Brian P McSharry; Simone K Forbes; Selmir Avdic; Richard E Randall; Gavin W G Wilkinson; Allison Abendroth; Barry Slobedman

doi:10.1128/JVI.02988-14

Abrogation of the interferon response promotes more efficient human cytomegalovirus replication

Brian P McSharry, Simone K Forbes, Selmir Avdic, Richard E Randall, Gavin W G Wilkinson, Allison Abendroth, Barry Slobedman

Dentistry and Medical Sciences

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

The effect of abrogating the interferon (IFN) response on human cytomegalovirus (HCMV) replication was investigated using primary human cells engineered to block either the production of or the response to type I IFNs. In IFN-deficient cells, HCMV produced larger plaques and spread and replicated more rapidly than in parental cells. These cells demonstrate the vital role of IFNs in controlling HCMV replication and provide useful tools to investigate the IFN response to HCMV.

Original language	English
Pages (from-to)	1479-1483
Number of pages	5
Journal	Journal of Virology
Volume	89
Issue number	2
DOIs	https://doi.org/10.1128/JVI.02988-14
Publication status	Published - 15 Jan 2015

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abstract = "The effect of abrogating the interferon (IFN) response on human cytomegalovirus (HCMV) replication was investigated using primary human cells engineered to block either the production of or the response to type I IFNs. In IFN-deficient cells, HCMV produced larger plaques and spread and replicated more rapidly than in parental cells. These cells demonstrate the vital role of IFNs in controlling HCMV replication and provide useful tools to investigate the IFN response to HCMV. ",

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AU - Wilkinson, Gavin W G

AU - Abendroth, Allison

AU - Slobedman, Barry

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AB - The effect of abrogating the interferon (IFN) response on human cytomegalovirus (HCMV) replication was investigated using primary human cells engineered to block either the production of or the response to type I IFNs. In IFN-deficient cells, HCMV produced larger plaques and spread and replicated more rapidly than in parental cells. These cells demonstrate the vital role of IFNs in controlling HCMV replication and provide useful tools to investigate the IFN response to HCMV.

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KW - Cytomegalovirus/immunology

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KW - Interferon Type I/deficiency

KW - Viral Plaque Assay

KW - Virus Replication

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Abrogation of the interferon response promotes more efficient human cytomegalovirus replication

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