Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.

K.G. Renz; B.F. Cheetham; A. Islam; S.W. Walkden-Brown

Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.

K.G. Renz, B.F. Cheetham, A. Islam, S.W. Walkden-Brown

Science and Health

Research output: Book chapter/Published conference paper › Conference paper › peer-review

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Abstract

Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.

Original language	English
Title of host publication	18th Annual Proceedings
Editors	T.A. Scott
Place of Publication	Sydney, Australia
Publisher	University of Sydney
Pages	153' 156.
Volume	18
Publication status	Published - 2006
Event	Australian Poultry Science Symposium (APSS) - Sydney, New South Wales, Australia Duration: 20 Feb 2006 → 22 Feb 2006

Conference

Conference	Australian Poultry Science Symposium (APSS)
Country/Territory	Australia
Period	20/02/06 → 22/02/06

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Absolute quantification of Marek’s disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.Accepted author manuscript, 274 KB

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title = "Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.",

abstract = "Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.",

keywords = "Open access version available",

author = "K.G. Renz and B.F. Cheetham and A. Islam and S.W. Walkden-Brown",

note = "Imported on 03 May 2017 - DigiTool details were: publisher = Sydney, Australia: University of Sydney, 2006. editor/s (773b) = Prof T A Scott; Event dates (773o) = 20-22 February 2006; Parent title (773t) = Australian Poultry Science Symposium (APSS). ISSNs: 1034-6260; ; Australian Poultry Science Symposium (APSS) ; Conference date: 20-02-2006 Through 22-02-2006",

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language = "English",

volume = "18",

pages = "153' 156.",

editor = "T.A. Scott",

booktitle = "18th Annual Proceedings",

publisher = "University of Sydney",

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Renz, KG, Cheetham, BF, Islam, A & Walkden-Brown, SW 2006, Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. in TA Scott (ed.), 18th Annual Proceedings. vol. 18, University of Sydney, Sydney, Australia, pp. 153' 156., Australian Poultry Science Symposium (APSS), Australia, 20/02/06.

Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. / Renz, K.G.; Cheetham, B.F.; Islam, A. et al.
18th Annual Proceedings. ed. / T.A. Scott. Vol. 18 Sydney, Australia: University of Sydney, 2006. p. 153' 156.

Research output: Book chapter/Published conference paper › Conference paper › peer-review

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T1 - Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.

AU - Renz, K.G.

AU - Cheetham, B.F.

AU - Islam, A.

AU - Walkden-Brown, S.W.

N1 - Imported on 03 May 2017 - DigiTool details were: publisher = Sydney, Australia: University of Sydney, 2006. editor/s (773b) = Prof T A Scott; Event dates (773o) = 20-22 February 2006; Parent title (773t) = Australian Poultry Science Symposium (APSS). ISSNs: 1034-6260;

PY - 2006

Y1 - 2006

N2 - Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.

AB - Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.

KW - Open access version available

M3 - Conference paper

VL - 18

SP - 153' 156.

BT - 18th Annual Proceedings

A2 - Scott, T.A.

PB - University of Sydney

CY - Sydney, Australia

T2 - Australian Poultry Science Symposium (APSS)

Y2 - 20 February 2006 through 22 February 2006

ER -

Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.

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