Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples.

K.G. Renz, B.F. Cheetham, A. Islam, S.W. Walkden-Brown

Research output: Book chapter/Published conference paperConference paper

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Abstract

Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.
Original languageEnglish
Title of host publication18th Annual Proceedings
EditorsT.A. Scott
Place of PublicationSydney, Australia
PublisherUniversity of Sydney
Pages153' 156.
Volume18
Publication statusPublished - 2006
EventAustralian Poultry Science Symposium (APSS) - Sydney, New South Wales, Australia
Duration: 20 Feb 200622 Feb 2006

Conference

ConferenceAustralian Poultry Science Symposium (APSS)
CountryAustralia
Period20/02/0622/02/06

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Polymerase chain reaction
Viruses
Dust
Assays
Farms

Cite this

Renz, K. G., Cheetham, B. F., Islam, A., & Walkden-Brown, S. W. (2006). Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. In T. A. Scott (Ed.), 18th Annual Proceedings (Vol. 18, pp. 153' 156.). Sydney, Australia: University of Sydney.
Renz, K.G. ; Cheetham, B.F. ; Islam, A. ; Walkden-Brown, S.W. / Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. 18th Annual Proceedings. editor / T.A. Scott. Vol. 18 Sydney, Australia : University of Sydney, 2006. pp. 153' 156.
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abstract = "Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.",
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Renz, KG, Cheetham, BF, Islam, A & Walkden-Brown, SW 2006, Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. in TA Scott (ed.), 18th Annual Proceedings. vol. 18, University of Sydney, Sydney, Australia, pp. 153' 156., Australian Poultry Science Symposium (APSS), Australia, 20/02/06.

Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. / Renz, K.G.; Cheetham, B.F.; Islam, A.; Walkden-Brown, S.W.

18th Annual Proceedings. ed. / T.A. Scott. Vol. 18 Sydney, Australia : University of Sydney, 2006. p. 153' 156.

Research output: Book chapter/Published conference paperConference paper

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N1 - Imported on 03 May 2017 - DigiTool details were: publisher = Sydney, Australia: University of Sydney, 2006. editor/s (773b) = Prof T A Scott; Event dates (773o) = 20-22 February 2006; Parent title (773t) = Australian Poultry Science Symposium (APSS). ISSNs: 1034-6260;

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AB - Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.

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Renz KG, Cheetham BF, Islam A, Walkden-Brown SW. Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. In Scott TA, editor, 18th Annual Proceedings. Vol. 18. Sydney, Australia: University of Sydney. 2006. p. 153' 156.