Methods for taqman real-time PCR assays to detect three serotypes of MDV are available (Islam et al., 2004), and an absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in this paper. Thus, it is now possible to perform qPCR assays for all three serotypes of MDV on a sample. Absolute quantification of MDV2 in dust samples from pulty farms across Australia in a preliminary study, revealed the prescence of MDV2 in 13 of 30 samples tested.
|Title of host publication||18th Annual Proceedings|
|Place of Publication||Sydney, Australia|
|Publisher||University of Sydney|
|Publication status||Published - 2006|
|Event||Australian Poultry Science Symposium (APSS) - Sydney, New South Wales, Australia|
Duration: 20 Feb 2006 → 22 Feb 2006
|Conference||Australian Poultry Science Symposium (APSS)|
|Period||20/02/06 → 22/02/06|
Renz, K. G., Cheetham, B. F., Islam, A., & Walkden-Brown, S. W. (2006). Absolute quantification of Marek's disease virus serotype 2 using real-time polymerase chain reaction and its application to field dust samples. In T. A. Scott (Ed.), 18th Annual Proceedings (Vol. 18, pp. 153' 156.). University of Sydney.