Sixty-seven residents of an area of China endemic for schistosomiasis (Schistosoma japonicum) were divided into 3 groups. The first was resistant (with high exposure level and negative stool for schistosome eggs; n=21), the 2nd was susceptible (positive stool; n=16) and the 3rd was indeterminate (with lower exposure level and negative stool; n=30). Peripheral blood mononuclear cells (PBMCs) from these subjects and from individuals residing in a non-endemic area (control group) were incubated in the presence of adult worm antigen (AWA), or 5 recombinant S. japonicum antigens. Cytokines (IL-5, IL-10 and IFN-γ) were determined quantitatively by sandwich-ELISA using cytokine-specific monoclonal antibodies. Following stimulation with AWA, PBMCs from individuals from the endemic area produced significantly higher levels of IL-5, IL-10 and IFN-γ than did those from controls. When PBMCs were incubated in the presence of one of the 5 recombinant schistosome antigens, only IL-10 production differed significantly between the endemic and non-endemic groups. Following stimulation by TegAg, FABP or GAPDH, PBMCs from individuals of the resistant group produced markedly higher levels of IL-10 than did those from the susceptible group. Following stimulation by GST [glutathione S-transferase], levels of IFN-γ in supernatants of PBMCs from resistant individuals were significantly higher than those of PBMCs from susceptible individuals. No direct evidence of a relationship between cytokine production and exposure levels was observed.
|Original language||Chinese (Traditional)|
|Number of pages||5|
|Journal||Chinese Journal of Schistosomiasis Control|
|Publication status||Published - 2000|