Acquisition of plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli of livestock origin in Bangladesh

Avijit Dutta, Md. Zohorul Islam, Himel Barua, Eaftekhar Ahmed Rana, Mohammad Shah Jalal, Pangkaj Kumar Dhar, Avijit Das, Tridip Das, Sabuj Manohar Sarma, Sanjoy Kanti Biswas, Paritosh Kumar Biswas

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Aims: To investigate plasmid-borne colistin resistance mechanism (plasmid-mediated colistin resistance [mcr-1]) in Escherichia coli of human, veterinary, and environmental origin in Bangladesh.

Materials and methods:
A total of 810 samples were collected from different sources. Isolation and identification of E. coli was performed using classical bacteriology and then tested for antimicrobial susceptibility. Colistin-resistant isolates were further analyzed for mcr-1 gene using PCR. Minimum inhibitory concentration (MIC) was determined using microbroth dilution technique. After sequencing of mcr-1 gene, phylogenetics was conducted to see the relationship with other mcr-1 gene sequences.

Results: A total of 358 E. coli were isolated from 810 samples of humans, animals, environment, and food in Bangladesh. Of them 49 (15.9%) isolates were phenotypically resistant to colistin and 254 (70.9%) were resistant to multiple antimicrobials. mcr-1 gene was detected in three E. coli isolates of poultry source. For the three mcr-1 positive isolates the MIC of colistin sulfate was 4, 8, and 128 μg/mL. Gene sequencing of two of the three mcr-1 positive isolates and phylogenetic analysis showed close similarities of one isolate to other mcr-1 sequences available at GenBank while the other appeared to have evolved locally.

Conclusion: First-ever report on circulation of mcr-1 E. coli of livestock origin in Bangladesh.
Original languageEnglish
Pages (from-to)1058-1062
Number of pages5
JournalMicrobial Drug Resistance
Volume26
Issue number9
DOIs
Publication statusPublished - 2020

Fingerprint

Dive into the research topics of 'Acquisition of plasmid-mediated colistin resistance gene mcr-1 in Escherichia coli of livestock origin in Bangladesh'. Together they form a unique fingerprint.

Cite this