Activation of innate immune-response genes in little brown bats (myotis lucifugus) infected with the fungus pseudogymnoascus destructans

Noreen Rapin, Kirk Johns, Lauren Martin, Lisa Warnecke, James M. Turner, Trent K. Bollinger, Craig K. R. Willis, Jamie Voyles, Vikram Misra

Research output: Contribution to journalArticle

15 Citations (Scopus)
1 Downloads (Pure)

Abstract

Recently bats have been associated with the emergence of diseases, both as reservoirs for several new viral diseases in humans and other animals and, in the northern Americas, as hosts for a devastating fungal disease that threatens to drive several bat species to regional extinction. However, despite these catastrophic events little Information is available on bat defences or how they interact with their pathogens. Even less is known about the response of bats to infection during torpor or long-term hibernation. Using tissue samples collected at the termination of an experiment to explore the pathogenesis of White Nose Syndrome in Little Brown Bats, we determined if hibernating bats infected with the fungus Pseudogymnoascus destructans could respond to infection by activating genes responsible for innate immune and stress responses. Lesions due to fungal infection and, in some cases, secondary bacterial infections, were restricted to the skin. However, we were unable to obtain sufficient amounts of RNA from these sites. We therefore examined lungs for response at an epithelial surface not linked to the primary site of infection. We found that bats responded to infection with a significant increase in lungs of transcripts for Cathelicidin (an anti-microbial peptide) as well as the immune modulators tumor necrosis factor alpha and interleukins 10 and 23. In conclusion, hibernating bats can respond to experimental P. destructans infection by activating expression of innate immune response genes.
Original languageEnglish
Pages (from-to)1-7
Number of pages7
JournalPLoS One
Volume9
Issue number11
DOIs
Publication statusPublished - 12 Nov 2014

Cite this

Rapin, Noreen ; Johns, Kirk ; Martin, Lauren ; Warnecke, Lisa ; Turner, James M. ; Bollinger, Trent K. ; Willis, Craig K. R. ; Voyles, Jamie ; Misra, Vikram. / Activation of innate immune-response genes in little brown bats (myotis lucifugus) infected with the fungus pseudogymnoascus destructans. In: PLoS One. 2014 ; Vol. 9, No. 11. pp. 1-7.
@article{7065bc95987e47a88cc479cf9f681e50,
title = "Activation of innate immune-response genes in little brown bats (myotis lucifugus) infected with the fungus pseudogymnoascus destructans",
abstract = "Recently bats have been associated with the emergence of diseases, both as reservoirs for several new viral diseases in humans and other animals and, in the northern Americas, as hosts for a devastating fungal disease that threatens to drive several bat species to regional extinction. However, despite these catastrophic events little Information is available on bat defences or how they interact with their pathogens. Even less is known about the response of bats to infection during torpor or long-term hibernation. Using tissue samples collected at the termination of an experiment to explore the pathogenesis of White Nose Syndrome in Little Brown Bats, we determined if hibernating bats infected with the fungus Pseudogymnoascus destructans could respond to infection by activating genes responsible for innate immune and stress responses. Lesions due to fungal infection and, in some cases, secondary bacterial infections, were restricted to the skin. However, we were unable to obtain sufficient amounts of RNA from these sites. We therefore examined lungs for response at an epithelial surface not linked to the primary site of infection. We found that bats responded to infection with a significant increase in lungs of transcripts for Cathelicidin (an anti-microbial peptide) as well as the immune modulators tumor necrosis factor alpha and interleukins 10 and 23. In conclusion, hibernating bats can respond to experimental P. destructans infection by activating expression of innate immune response genes.",
keywords = "Bats, Immune resoponse, Hibernation, Fungal diseases, Wings, Neutrophils, Cytokines, Polymerase chain reaction",
author = "Noreen Rapin and Kirk Johns and Lauren Martin and Lisa Warnecke and Turner, {James M.} and Bollinger, {Trent K.} and Willis, {Craig K. R.} and Jamie Voyles and Vikram Misra",
note = "Includes bibliographical references.",
year = "2014",
month = "11",
day = "12",
doi = "10.1371/journal.pone.0112285",
language = "English",
volume = "9",
pages = "1--7",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science (PLoS)",
number = "11",

}

Activation of innate immune-response genes in little brown bats (myotis lucifugus) infected with the fungus pseudogymnoascus destructans. / Rapin, Noreen; Johns, Kirk; Martin, Lauren; Warnecke, Lisa; Turner, James M.; Bollinger, Trent K.; Willis, Craig K. R.; Voyles, Jamie; Misra, Vikram.

In: PLoS One, Vol. 9, No. 11, 12.11.2014, p. 1-7.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Activation of innate immune-response genes in little brown bats (myotis lucifugus) infected with the fungus pseudogymnoascus destructans

AU - Rapin, Noreen

AU - Johns, Kirk

AU - Martin, Lauren

AU - Warnecke, Lisa

AU - Turner, James M.

AU - Bollinger, Trent K.

AU - Willis, Craig K. R.

AU - Voyles, Jamie

AU - Misra, Vikram

N1 - Includes bibliographical references.

PY - 2014/11/12

Y1 - 2014/11/12

N2 - Recently bats have been associated with the emergence of diseases, both as reservoirs for several new viral diseases in humans and other animals and, in the northern Americas, as hosts for a devastating fungal disease that threatens to drive several bat species to regional extinction. However, despite these catastrophic events little Information is available on bat defences or how they interact with their pathogens. Even less is known about the response of bats to infection during torpor or long-term hibernation. Using tissue samples collected at the termination of an experiment to explore the pathogenesis of White Nose Syndrome in Little Brown Bats, we determined if hibernating bats infected with the fungus Pseudogymnoascus destructans could respond to infection by activating genes responsible for innate immune and stress responses. Lesions due to fungal infection and, in some cases, secondary bacterial infections, were restricted to the skin. However, we were unable to obtain sufficient amounts of RNA from these sites. We therefore examined lungs for response at an epithelial surface not linked to the primary site of infection. We found that bats responded to infection with a significant increase in lungs of transcripts for Cathelicidin (an anti-microbial peptide) as well as the immune modulators tumor necrosis factor alpha and interleukins 10 and 23. In conclusion, hibernating bats can respond to experimental P. destructans infection by activating expression of innate immune response genes.

AB - Recently bats have been associated with the emergence of diseases, both as reservoirs for several new viral diseases in humans and other animals and, in the northern Americas, as hosts for a devastating fungal disease that threatens to drive several bat species to regional extinction. However, despite these catastrophic events little Information is available on bat defences or how they interact with their pathogens. Even less is known about the response of bats to infection during torpor or long-term hibernation. Using tissue samples collected at the termination of an experiment to explore the pathogenesis of White Nose Syndrome in Little Brown Bats, we determined if hibernating bats infected with the fungus Pseudogymnoascus destructans could respond to infection by activating genes responsible for innate immune and stress responses. Lesions due to fungal infection and, in some cases, secondary bacterial infections, were restricted to the skin. However, we were unable to obtain sufficient amounts of RNA from these sites. We therefore examined lungs for response at an epithelial surface not linked to the primary site of infection. We found that bats responded to infection with a significant increase in lungs of transcripts for Cathelicidin (an anti-microbial peptide) as well as the immune modulators tumor necrosis factor alpha and interleukins 10 and 23. In conclusion, hibernating bats can respond to experimental P. destructans infection by activating expression of innate immune response genes.

KW - Bats

KW - Immune resoponse

KW - Hibernation

KW - Fungal diseases

KW - Wings

KW - Neutrophils

KW - Cytokines

KW - Polymerase chain reaction

U2 - 10.1371/journal.pone.0112285

DO - 10.1371/journal.pone.0112285

M3 - Article

VL - 9

SP - 1

EP - 7

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

ER -