Neural tension has been proposed to be a factor influencing multi-joint movements such as sprinting, kicking and bending to pick up an object. Neural mobilizations have been demonstrated to increase range of motion in one joint, however the effect on flexibility across multiple joints has not been described nor compared to the traditional static stretch response. The aim of this study was to compare the effect on flexibility across multiple joints of neural mobilization to the traditional static stretch response. Fifty-two young adults (F = 32, M = 20; aged 18 ' 25 years) were recruited from Charles Sturt University and a NE Victorian cross country ski camp and randomly allocated to receive a neural mobilization or static hamstring stretching intervention. The neural mobilization group received three, thirty-second passive Grade III neural mobilizations and the static stretch group received three, thirty-second passive static hamstring stretches. Effects of intervention were evaluated using the Mann-Whitney U test for unmatched samples. Pre-post difference in flexibility/range of motion was assessed using the Wilcoxon Signed Ranks test for matched samples. Spearman's Rank Order Correlation analysis was performed to assess correlations between participant characteristics and the change in flexibility following intervention. Post-intervention toe touch distance increased significantly following neural mobilization (median change = 22.5 mm; p < 0.01) and statichamstring stretching (median change = 25.0 mm; p < 0.01). There was no significant difference between the effects of either intervention on toe touch distance. A single session of neural mobilization produces a similar increase in toe touch distance to static hamstring range of motion, suggesting that neural tension may be a factor influencing multijoint range of motion.