Adenovirus vector delivery stimulates natural killer cell recognition

Peter Tomasec, Eddie C Y Wang, Veronika Groh, Thomas Spies, Brian P McSharry, Rebecca J Aicheler, Richard J Stanton, Gavin W G Wilkinson

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

We report that delivery of first-generation replication-deficient adenovirus (RDAd) vectors into primary human fibroblasts is associated with the induction of natural killer (NK) cell-mediated cytolysis in vitro. RDAd vector delivery induced cytolysis by a range of NK cell populations including the NK cell clone NKL, primary polyclonal NK lines and a proportion of NK clones (36 %) in autologous HLA-matched assays. Adenovirus-induced cytolysis was inhibited by antibody blocking of the NK-activating receptor NKG2D, implicating this receptor in this function. NKG2D is ubiquitously expressed on NK cells and CD8+ T cells. Significantly, γ-irradiation of the vector eliminated the effect, suggesting that breakthrough expression from the vector induces at least some of the pro-inflammatory responses of unknown aetiology following the application of RDAd vectors during in vivo gene delivery.

Original languageEnglish
Pages (from-to)1103-1108
Number of pages6
JournalJournal of General Virology
Volume88
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Dive into the research topics of 'Adenovirus vector delivery stimulates natural killer cell recognition'. Together they form a unique fingerprint.

Cite this