Allelic variations in high and low molecular weight glutenins at the Glu-Dt locus of Aegilops tauschii as a potential source for improving bread wheat quality

A. Rehman, N. Evans, M. C. Gianibelli, R. J. Rose

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Aegilops tauschii, the donor of the D genome of hexaploid wheat, is accepted as a major contributor of disease resistance and bread-making quality attributes in cultivated wheat. High molecular weight (HMW) glutenins have a significant effect on the bread-making qualities of cultivated wheat. A large range of allelic variation in 424 Ae. tauschii accessions at the Glu-D1 t locus for both x- and y-type glutenin subunits as well as at Glu-D3t was observed with SDS-PAGE using the total endosperm protein fraction. Only 4 accessions revealed more than 2 bands on SDS-PAGE. Seventeen new allelic combinations of both x- and y-type glutenin subunits at GluD-1 t and 30 new allelic profiles at Glu-D3 were detected. These combinations comprise some lines with x- or y-type null forms. RP-HPLC analysis of accession Aus 18882, which showed 5 bands when the total endosperm protein fraction was resolved on SDS-PAGE, revealed 2 x-types and 1 y-type subunit banding pattern. RP-HPLC of the gliadin fraction exhibited an omega gliadin-like subunit. SDS-PAGE processing of the gliadin-free fraction of the same accession still exhibited the gliadin-like protein. An N-terminal protein sequence of the first 7 amino acids of the slowest moving novel x-type band of accession Aus18882 indicated its uniqueness, as no entries were found to contain this internal sequence. Demonstration of novel allelic combinations at Glu-D1 and Glu-D3 loci implies the potential for exploiting Ae. tauschii to increase the genetic variability of hexaploid wheat, particularly for bread-making qualities.

Original languageEnglish
Pages (from-to)399-405
Number of pages7
JournalAustralian Journal of Agricultural Research
Volume59
Issue number5
DOIs
Publication statusPublished - 20 May 2008

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Gliadin
Aegilops tauschii
gliadin
Bread
glutenins
Triticum
Polyacrylamide Gel Electrophoresis
Molecular Weight
molecular weight
Endosperm
loci
wheat
hexaploidy
breads
endosperm
Proteins
High Pressure Liquid Chromatography
Disease Resistance
proteins
disease resistance

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title = "Allelic variations in high and low molecular weight glutenins at the Glu-Dt locus of Aegilops tauschii as a potential source for improving bread wheat quality",
abstract = "Aegilops tauschii, the donor of the D genome of hexaploid wheat, is accepted as a major contributor of disease resistance and bread-making quality attributes in cultivated wheat. High molecular weight (HMW) glutenins have a significant effect on the bread-making qualities of cultivated wheat. A large range of allelic variation in 424 Ae. tauschii accessions at the Glu-D1 t locus for both x- and y-type glutenin subunits as well as at Glu-D3t was observed with SDS-PAGE using the total endosperm protein fraction. Only 4 accessions revealed more than 2 bands on SDS-PAGE. Seventeen new allelic combinations of both x- and y-type glutenin subunits at GluD-1 t and 30 new allelic profiles at Glu-D3 were detected. These combinations comprise some lines with x- or y-type null forms. RP-HPLC analysis of accession Aus 18882, which showed 5 bands when the total endosperm protein fraction was resolved on SDS-PAGE, revealed 2 x-types and 1 y-type subunit banding pattern. RP-HPLC of the gliadin fraction exhibited an omega gliadin-like subunit. SDS-PAGE processing of the gliadin-free fraction of the same accession still exhibited the gliadin-like protein. An N-terminal protein sequence of the first 7 amino acids of the slowest moving novel x-type band of accession Aus18882 indicated its uniqueness, as no entries were found to contain this internal sequence. Demonstration of novel allelic combinations at Glu-D1 and Glu-D3 loci implies the potential for exploiting Ae. tauschii to increase the genetic variability of hexaploid wheat, particularly for bread-making qualities.",
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Allelic variations in high and low molecular weight glutenins at the Glu-Dt locus of Aegilops tauschii as a potential source for improving bread wheat quality. / Rehman, A.; Evans, N.; Gianibelli, M. C.; Rose, R. J.

In: Australian Journal of Agricultural Research, Vol. 59, No. 5, 20.05.2008, p. 399-405.

Research output: Contribution to journalArticle

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AU - Rehman, A.

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