TY - JOUR
T1 - An electrochemical method for sensitive and rapid detection of FAM134B protein in colon cancer samples
AU - Islam, Farhadul
AU - Haque, Md Hakimul
AU - Yadav, Sharda
AU - Islam, Md Nazmul
AU - Gopalan, Vinod
AU - Nguyen, Nam Trung
AU - Lam, Alfred K.
AU - Shiddiky, Muhammad J.A.
N1 - Funding Information:
This work was supported by the NHMRC CDF (APP1088966 to M.J.A.S.), 2016 Griffith University new researcher grant scheme, 2015 MBoD strategic seed grant of Menzies Health Institute Queensland, higher degree research scholarships (GUIPRS and GUPRS scholarships to F.I., M.H.H., S.Y., M.N.I.).
Publisher Copyright:
© The Author(s) 2017.
PY - 2017/3/9
Y1 - 2017/3/9
N2 - Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3−/4− redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL−1 in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.
AB - Despite the excellent diagnostic applications of the current conventional immunoassay methods such as ELISA, immunostaining and Western blot for FAM134B detection, they are laborious, expensive and required a long turnaround time. Here, we report an electrochemical approach for rapid, sensitive, and specific detection of FAM134B protein in biological (colon cancer cell extracts) and clinical (serum) samples. The approach utilises a differential pulse voltammetry (DPV) in the presence of the [Fe(CN)6]3−/4− redox system to quantify the FAM134B protein in a two-step strategy that involves (i) initial attachment of FAM134B antibody on the surface of extravidin-modified screen-printed carbon electrode, and (ii) subsequent detection of FAM134B protein present in the biological/clinical samples. The assay system was able to detect FAM134B protein at a concentration down to 10 pg μL−1 in phosphate buffered saline (pH 7.4) with a good inter-assay reproducibility (% RSD = <8.64, n = 3). We found excellent sensitivity and specificity for the analysis of FAM134B protein in a panel of colon cancer cell lines and serum samples. Finally, the assay was further validated with ELISA method. We believe that our assay could potentially lead a low-cost alternative to conventional immunological assays for target antigens analysis in point-of-care applications.
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U2 - 10.1038/s41598-017-00206-8
DO - 10.1038/s41598-017-00206-8
M3 - Article
C2 - 28273937
AN - SCOPUS:85026203621
SN - 2045-2322
VL - 7
SP - 1
EP - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
M1 - 133
ER -
