An update on using CRISPR/Cas9 in the one-cell stage mouse embryo for generating complex mutant alleles

Andrew J Kueh, Martin Pal, Lin Tai, Yang Liao, Gordon K Smyth, Wei Shi, Marco J Herold

Research output: Contribution to journalArticlepeer-review

20 Citations (Scopus)

Abstract

The generation of knockout mouse models is essential for understanding complex biological pathways within the whole organism. Elucidating cell death mechanisms in vivo or identifying cancer vulnerabilities in a model system has only been possible through the generation of knockout mice lacking the genes thought to be involved in these processes.1–3 However, the constitutive deletion of certain gene products, such as MCL-1 for example, often results in early embryonic lethality. 4 Therefore,conditional deletion methodologies have been invaluable for analyzing the phenotype of genes critical for embryonic development in the adult mouse. However, generation of mice with conditional alleles or other complex genetic alterations has to date been both very difficult and labor intense
Original languageEnglish
Pages (from-to)1821-1822
Number of pages2
JournalCell Death and Differentiation
Volume24
Issue number10
DOIs
Publication statusPublished - Oct 2017

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