Analysis of volatile compunds in hydraulic pressed camellia oil by SPME-GC-MS method (in Chinese)

A method for volatile analysis of hydraulic pressed camellia oil (Camellia oleifera Abel) by headspace solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME-GC-MS) using 50/30 'm DVB/CAR/PDMS fiber head is presented. Calibration curves were constructed for 13 compounds commonly reported in camellia oil headspace with good leaner relationship, correlation coefficients (R2) ranging from 0.9841 to 0.9999 and detection limits from 0.04 'g/g to 0.44 'g/g. The recoveries and relatives standard deviation in repetitive determination were ranged from 91.83% to 105.11% and 2.51% to 8.00%, respectively. 37 volatile compounds in hydraulic pressed camellia oil were identified by mass spectrum and standard retention time, which included aldehydes, alcohols, ketons, esters and acids etc. The total concentration of the top ten compounds, namely, hexanal, '-pinene, ethyl acetate, pentanol, benzaldehyde, octanol, '-Muurolene, nonanol, decanol, and octanal, was reached to 21.26 'g/g and accounted for 68.40% of total. These data indicate that this method is rapid, accurate and convenient for volatile analysis of camellia oil.