In this study the activity of 13 honeys, including three commercial antibacterial honeys, against Escherichia coli and Pseudomonas aeruginosa was determined. Antibacterial activity of the honeys was assayed using standard well diffusion methods. All honeys, and an artificial honey, were tested at four concentrations (10%, 5%, 2.5%, and 1% wt/vol) against E. coli and P. aeruginosa, and zones of inhibition were measured. All honeys tested had an inhibitory effect on the growth of E. coli and P. aeruginosa, with one honey still having activity against E. coli and three having activity against P. aeruginosa at 2.5%. No honey was active at 1% concentrations. E. coli was more susceptible to inhibition by the honeys used in this study than was P. aeruginosa. In this study we have demonstrated that several honeys, in addition to commercial antibacterial honeys, can inhibit E. coli and P. aeruginosa and may have potential as therapeutic honeys.