Antibacterial activity of fresh raw milk against Listeria monocytogenes

Wayne Pitt, Terence Harden

Research output: Book chapter/Published conference paperConference paperpeer-review

Abstract

With increasing pressures to use raw milk for the manufacture of processed dairy products, studies were undertaken to determine the feasibility of exploiting the natural antimicrobial systems of fresh raw milk to inhibit the growth of Listeria monocytogenes which has been cited as the cause of listeriosis following the consumption of contaminated milk and dairy products. High-Temperature Short-Time pasteurized milk and raw milk stored for 0 (fresh raw milk), 4 and 6 days at 4°C were inoculated to contain an initial population of approximately 103-104 cfu/mL of L. monocytogenes. Test samples and controls were incubated at temperatures in the range 4°C-37°C and the growth rate and pH were determined at regular intervals for up to 12 days. Fresh raw milk was found to inhibit the growth of L. monocytogenes slightly at 25°C but markedly at 37°C, with the pathogen becoming undetectable in fresh raw milk after 56 hours incubation at 37°C. No inhibition of L. monocytogenes was observed at 4°C or 7°C, and the inhibitory effect of raw milk against L. monocytogenes decreased with increasing duration of milk storage. Tests were performed in duplicate and control samples were negative for the growth of L. monocytogenes. The ability of fresh raw milk to inhibit the growth of L. monocytogenes at 37°C and the potential use of such milk for the production of cheese which is safe for human consumption requires further study.
Original languageEnglish
Title of host publicationBiotechnology
Subtitle of host publicationFood and Health for the 21st Century
EditorsAustralian Biotechnology Association
Pages151
Number of pages1
Publication statusPublished - 1998
Event14th Australasian Biotechnology Conference - Glenelg, Adelaide, South Australia, Australia
Duration: 19 Apr 199823 Apr 1998

Conference

Conference14th Australasian Biotechnology Conference
Country/TerritoryAustralia
Period19/04/9823/04/98

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