Antifibrotic effect of Smad decoy oligodeoxynucleotide in a CCl4-induced hepatic fibrosis animal model

Mi Gyeong Gwon, Jung Yeon Kim, Hyun Jin An, Woon Hae Kim, Hyemin Gu, Min Kyung Kim, Sok Cheon Pak, Kwan Kyu Park

Research output: Contribution to journalArticle

2 Citations (Scopus)
3 Downloads (Pure)

Abstract

Hepatic fibrosis is the wound-healing process of chronic hepatic disease that leads to the end-stage of hepatocellular carcinoma and demolition of hepatic structures. Epithelial-mesenchymal transition (EMT) has been identified to phenotypic conversion of the epithelium to mesenchymal phenotype that occurred during fibrosis. Smad decoy oligodeoxynucleotide (ODN) is a synthetic DNA fragment containing a complementary sequence of Smad transcription factor. Thus, this study evaluated the antifibrotic effects of Smad decoy ODN on carbon tetrachloride (CCl4)-induced hepatic fibrosis in mice. As shown in histological results, CCl4 treatment triggered hepatic fibrosis and increased Smad expression. On the contrary, Smad decoy ODN administration suppressed fibrogenesis and EMT process. The expression of Smad signaling and EMT-associated protein was markedly decreased in Smad decoy ODN-treated mice compared with CCl4-injured mice. In conclusion, these data indicate the practicability of Smad decoy ODN administration for preventing hepatic fibrosis and EMT processes.

Original languageEnglish
Article number1991
Pages (from-to)1-13
Number of pages13
JournalMolecules
Volume23
Issue number8
DOIs
Publication statusPublished - 10 Aug 2018

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