Oxidative stress, inflammation, and endothelial dysfunction have been associated with the development of cardiovascular diseases. Rice bran (RB) derived phenolic extracts are recognised to have antioxidant and anti-inflammatory potential. This study aimed to evaluate if RB derived phenolic extracts could potentially modulate genes associated with antioxidant and anti-inflammatory pathways in human umbilical vein endothelial cells (HUVECs) under induced oxidative stress conditions. HUVECs were pre-treated with varying concentrations of RB phenolic extracts (25 – 250 µg/mL) and induced with oxidative stress environment. The expression of genes involved in the regulation of antioxidant and anti-inflammatory pathways was evaluated using quantitative real-time polymerase chain reaction. RB derived phenolic extracts downregulated the expression of ICAM-1, CD39, CD73, and NOX4 genes and up-regulated Nrf2, NQO1, HO1, and eNOS genes. The results support the hypothesis that RB derived phenolic extracts may potentially target genes associated with antioxidant and anti-inflammatory pathways in HUVECs induced with oxidative stress conditions.
|Publication status||Published - 27 Aug 2019|
|Event||69th Australasian Grain Science Conference - Rydges on Swanston, Melbourne, Australia|
Duration: 27 Aug 2019 → 29 Aug 2019
Conference number: 69
|Conference||69th Australasian Grain Science Conference|
|Abbreviated title||Grains: A global food resource|
|Period||27/08/19 → 29/08/19|