Apoptotic effect of MG-132 on human tongue squamous cell carcinoma

Bin Zhang, Hai-ying Chen, Shuang-feng Chen, Wei Liu, Wei-hua Wang, Xian-bin Liu, Ying-xin Zhang, Lexin Wang

Research output: Contribution to journalArticle

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Abstract

The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 'M of MG-132, or 5 'M thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18 h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30 'M MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin (8.5%, P < 0.01) or control group (0.5%, P < 0.01). The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30 'M MG-132 group was 28.75 ± 2.28, 18.16 ± 0.65, 8.85 ± 0.72, respectively, which was lower than in the thapsigargin (38.96 ± 0.33, P < 0.05 or 0.01) or control (40.88 ± 4.52, P < 0.05 or 0.01) group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group (P < 0.01). In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12.
Original languageEnglish
Pages (from-to)322-327
Number of pages6
JournalBiomedicine and Pharmacotherapy
Volume65
Issue number5
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Tongue
Squamous Cell Carcinoma
Caspase 12
Thapsigargin
Apoptosis
Ubiquitin-Protein Ligases
Control Groups
Messenger RNA
Proteins
Proteasome Inhibitors
benzyloxycarbonylleucyl-leucyl-leucine aldehyde
Propidium
Annexin A5
Ligases
Up-Regulation
Down-Regulation
Western Blotting
Enzyme-Linked Immunosorbent Assay
Staining and Labeling
Cell Line

Cite this

Zhang, Bin ; Chen, Hai-ying ; Chen, Shuang-feng ; Liu, Wei ; Wang, Wei-hua ; Liu, Xian-bin ; Zhang, Ying-xin ; Wang, Lexin. / Apoptotic effect of MG-132 on human tongue squamous cell carcinoma. In: Biomedicine and Pharmacotherapy. 2011 ; Vol. 65, No. 5. pp. 322-327.
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title = "Apoptotic effect of MG-132 on human tongue squamous cell carcinoma",
abstract = "The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 'M of MG-132, or 5 'M thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18 h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30 'M MG-132 group was 13.5, 19.6 and 34.7{\%}, respectively, which was higher than in the thapsigargin (8.5{\%}, P < 0.01) or control group (0.5{\%}, P < 0.01). The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30 'M MG-132 group was 28.75 ± 2.28, 18.16 ± 0.65, 8.85 ± 0.72, respectively, which was lower than in the thapsigargin (38.96 ± 0.33, P < 0.05 or 0.01) or control (40.88 ± 4.52, P < 0.05 or 0.01) group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group (P < 0.01). In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12.",
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Zhang, B, Chen, H, Chen, S, Liu, W, Wang, W, Liu, X, Zhang, Y & Wang, L 2011, 'Apoptotic effect of MG-132 on human tongue squamous cell carcinoma', Biomedicine and Pharmacotherapy, vol. 65, no. 5, pp. 322-327. https://doi.org/10.1016/j.biopha.2011.04.009

Apoptotic effect of MG-132 on human tongue squamous cell carcinoma. / Zhang, Bin; Chen, Hai-ying; Chen, Shuang-feng; Liu, Wei; Wang, Wei-hua; Liu, Xian-bin; Zhang, Ying-xin; Wang, Lexin.

In: Biomedicine and Pharmacotherapy, Vol. 65, No. 5, 08.2011, p. 322-327.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Apoptotic effect of MG-132 on human tongue squamous cell carcinoma

AU - Zhang, Bin

AU - Chen, Hai-ying

AU - Chen, Shuang-feng

AU - Liu, Wei

AU - Wang, Wei-hua

AU - Liu, Xian-bin

AU - Zhang, Ying-xin

AU - Wang, Lexin

N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = August, 2011; Journal title (773t) = Biomedicine and Pharmacotherapy. ISSNs: 0753-3322;

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N2 - The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 'M of MG-132, or 5 'M thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18 h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30 'M MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin (8.5%, P < 0.01) or control group (0.5%, P < 0.01). The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30 'M MG-132 group was 28.75 ± 2.28, 18.16 ± 0.65, 8.85 ± 0.72, respectively, which was lower than in the thapsigargin (38.96 ± 0.33, P < 0.05 or 0.01) or control (40.88 ± 4.52, P < 0.05 or 0.01) group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group (P < 0.01). In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12.

AB - The aim of this study was to investigate the apoptotic effect of a proteasome inhibitor MG-132 on Tca-8113, a cell line of human tongue squamous cell carcinoma. Tca-8113 cells were treated with 10, 20, and 30 'M of MG-132, or 5 'M thapsigargin. Apoptosis rate was determined with annexin V/propidium iodide double staining. Expression of E3ubiquitin-protein ligase was determined by ELISA, and Grp78 and caspase-12 mRNA, and Grp78 and caspase-12 protein was assessed by RT-PCR and Western blot, respectively. Apoptosis was observed 18 h after MG-132 treatment. The apoptotic rate in the 10, 20, and 30 'M MG-132 group was 13.5, 19.6 and 34.7%, respectively, which was higher than in the thapsigargin (8.5%, P < 0.01) or control group (0.5%, P < 0.01). The expression of E3 ubiquitin-protein ligase in the 10, 20, and 30 'M MG-132 group was 28.75 ± 2.28, 18.16 ± 0.65, 8.85 ± 0.72, respectively, which was lower than in the thapsigargin (38.96 ± 0.33, P < 0.05 or 0.01) or control (40.88 ± 4.52, P < 0.05 or 0.01) group. The levels of Grp78 and capase-12 mRNA, Grp78 and caspase-12 protein in the MG-132 groups were higher than in the control group (P < 0.01). In conclusion, MG-132 induces apoptosis in Tca-8113 cells in a concentration-dependent manner. The MG-132-induced apoptosis may involve downregulation of E3 ubiquitin ligase, and upregulation of Grp78 and caspase-12.

KW - 132

KW - Apoptosis

KW - E3 ubiquitin ligases

KW - Squamous cell carcinoma

KW - Tca-8113 cells

U2 - 10.1016/j.biopha.2011.04.009

DO - 10.1016/j.biopha.2011.04.009

M3 - Article

VL - 65

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EP - 327

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SN - 0753-3322

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