Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality

J. M.  Hoban; D. L.  Hopkins; N. Kirby; D. Collins; F. R. Dunshea; M. G. Kerr; K. Bailes; J.J. Cottrell; B. W. B.  Holman; W. Brown; E.N. Ponnampalam

doi:10.1016/j.meatsci.2016.03.005

Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality

J. M. Hoban, D. L. Hopkins, N. Kirby, D. Collins, F. R. Dunshea, M. G. Kerr, K. Bailes, J.J. Cottrell, B. W. B. Holman, W. Brown, E.N. Ponnampalam

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n = 50) and sheep (n = 50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force,intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5 day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.

Original language	English
Pages (from-to)	122-129
Number of pages	8
Journal	Meat Science
Volume	117
DOIs	https://doi.org/10.1016/j.meatsci.2016.03.005
Publication status	Published - Jul 2016

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@article{cd9277ad41a04b33b277caa017c967b8,

title = "Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality",

abstract = "A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n = 50) and sheep (n = 50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force,intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5 day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.",

keywords = "Intramuscular fat content, Lamb, Meat quality, Particle size analysis, Sarcomere length, Shear force, X-ray diffraction",

author = "Hoban, {J. M.} and Hopkins, {D. L.} and N. Kirby and D. Collins and Dunshea, {F. R.} and Kerr, {M. G.} and K. Bailes and J.J. Cottrell and Holman, {B. W. B.} and W. Brown and E.N. Ponnampalam",

note = "Includes bibliographical references.",

year = "2016",

month = jul,

doi = "10.1016/j.meatsci.2016.03.005",

language = "English",

volume = "117",

pages = "122--129",

journal = "Meat Science",

issn = "0309-1740",

publisher = "Elsevier",

}

TY - JOUR

T1 - Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality

AU - Hoban, J. M.

AU - Hopkins, D. L.

AU - Kirby, N.

AU - Collins, D.

AU - Dunshea, F. R.

AU - Kerr, M. G.

AU - Bailes, K.

AU - Cottrell, J.J.

AU - Holman, B. W. B.

AU - Brown, W.

AU - Ponnampalam, E.N.

N1 - Includes bibliographical references.

PY - 2016/7

Y1 - 2016/7

N2 - A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n = 50) and sheep (n = 50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force,intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5 day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.

AB - A small angle X-ray scattering (SAXS) synchrotron was used to evaluate 100 ovine m. longissimus lumborum, representing lamb (n = 50) and sheep (n = 50). The diffraction of X-rays gives information on muscle myofibril structure and fat content. The linear relationships between SAXS measures with measures such as, shear force,intramuscular fat content (IMF) and collagen content/solubility, were investigated. A relationship was found between the d-spacing of the actin/myosin fibril spacing (SAX1 and SAX2) and the cross sectional area of the rhombohedral unit cell (Cell area) and shear force after 1 and 5 day ageing. There was a positive relationship between IMF and a SAXS Fat area measure. There was a muscle site effect on SAX1, SAX2 and Cell area, with the cranial site having a larger distance between myofibrils. The potential of SAXS as a powerful research tool to determine not only the structural components of ovine tenderness, but also the fat content related to IMF is evident.

KW - Intramuscular fat content

KW - Lamb

KW - Meat quality

KW - Particle size analysis

KW - Sarcomere length

KW - Shear force

KW - X-ray diffraction

U2 - 10.1016/j.meatsci.2016.03.005

DO - 10.1016/j.meatsci.2016.03.005

M3 - Article

C2 - 26971308

SN - 0309-1740

VL - 117

SP - 122

EP - 129

JO - Meat Science

JF - Meat Science

ER -

Application of small angle X-ray scattering synchrotron technology for measuring ovine meat quality

Abstract

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