Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability

Young Hoon Bai, Sokcheon Pak, Seung Hoo Lee, Chun Sik Bae, Colin Prosser, Kerst Stelwagen, Jin Hee Lee, Soon Duck Park

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Lactobacillus probiotic strains are proving to be abundant sources of bioactive components, including antiinflammatory components. Lifree was made of fruits fermented by Lactobacillus paracasei, Lactobacillus reuterrii and Saccharomyces cerevisiae. This study was designed to test these compounds in cell assays measuring epithelial barrier function and proliferation in the first instance. Cell proliferation was measured in mouse fibroblasts cells (3T3NIH) and rat intestinal epithelial cells (IEC-6), and tight junction activity in the kidney epithelial cell line (MDCK). Tight junction permeability was assessed by measuring transepithelial electrical resistance (TER) across confluent monolayers, following the addition of Lifree with or without a challenge with EGTA. Lifree promoted tight junction formation and recovery following loss of TER from challenge with EGTA. On the other hand, Lifree did not stimulate cell growth in either 3T3NIH and IEC-6 cells. Lifree stimulates tight junction maintenance and formation, suggesting it may have potential antiinflammatory properties.
Original languageEnglish
Pages (from-to)1009-1012
Number of pages4
JournalPhytotherapy Research
Volume19
Issue number12
DOIs
Publication statusPublished - 2005

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    Bai, Y. H., Pak, S., Lee, S. H., Bae, C. S., Prosser, C., Stelwagen, K., Lee, J. H., & Park, S. D. (2005). Assessment of a bioactive compound for its potential antiinflammatory property by tight junction permeability. Phytotherapy Research, 19(12), 1009-1012. https://doi.org/10.1002/ptr.1772