Assessment of cytokine mRNA expression in bronchoalveolar lavage fluid cells of horses with inflammatory airway disease: A pilot study

Kristopher Hughes, L. Nicolson, N. Da Costa, S. Franklin, K. Allen, S. Dunham

Research output: Book chapter/Published conference paperConference paperpeer-review

Abstract

Aims: To investigate cytokine mRNA expression inbronchoalveolar cells of horses with inflammatory airway disease(IAD) and horses without lower airway disease. Methods:Bronchoalveolar lavage fluid (BALF) was collected from athletichorses during clinical investigation of poor performance. Basedon BALF cytology, horses were assigned to case (>5% neutrophilsand/or '5% mast cells) or control (<5% neutrophils, <5% mastcells) groups. Quantitative reverse transcriptase PCR assays wereperformed on cell pellets extracted from each BALF sample fordetermination of mRNA expression of interleukin (IL)-1ß, IL-2, IL-4, IL-8, IL-13, IL-17, tumour necrosis factor ' (TNF-') andinterferon-' (IFN-'). ß-actin mRNA expression was measured todetermine the relative expression for each gene of interest.Expression of cytokine mRNA was compared between groupsusing the Mann-Whitney rank sum test or t test on normallydistributed data following log transformation. Results: BALF wasobtained from 15 horses with IAD (age 7.53 ± 3.91 years; range2'15) and 15 control horses (5.73 ± 2.19; range 2'11). There wassignificantly greater mRNA expression of IL-1ß, IL-23 and TNF-'in horses with IAD than in the control group (P<0.05). There wasa trend for increased expression of IL-8 and IL-17 mRNA in horseswith IAD; however, this was not significant (P>0.05). Conclusionsand Practical Significance: The greater expression of IL-1ß, IL-23 and TNF-' mRNA in horses with IAD is consistent with upregulationof inflammatory processes and recruitment ofinflammatory cells to the airways. IL-1ß, IL-23 and TNF-' are proinflammatorycytokines and representative of non-specificinflammation. The lack of difference in the expression of IL-2, IFN-', IL-4 or IL-13 between case and control horses suggests apolarised T helper cell response is not involved in the pathogenesisof IAD in athletic horses. Acknowledgements: Funding wasprovided by Petplan Charitable Trust and Faculty of VeterinaryMedicine, University of Glasgow.
Original languageEnglish
Title of host publicationProceedings 48th BEVA
Subtitle of host publicationCardio Respiratory Medicine
PublisherEquine Veterinary Journal Ltd, Newmarket
Pages170-171
Number of pages2
Publication statusPublished - 2009
Event48th British Equine Veterinary Association Congress BEVA 2009 - Birmingham, United Kingdom, United Kingdom
Duration: 09 Sep 200912 Sep 2009

Conference

Conference48th British Equine Veterinary Association Congress BEVA 2009
CountryUnited Kingdom
Period09/09/0912/09/09

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