Abstract
The relative sensitivity of conventional assays of liver function was evaluated in comparison with galactose clearance, a test of liver function used in humans since the 1960s. Results of galactose clearance tests were compared with plasma enzyme concentrations and serum bile acid levels in clinically normal galahs (Eolophus roseicapillus; n = 8), after celiotomy (n = 4), and after 6% or 18% hepatectomy (n = 8, respectively). Clearance tests and biochemical analyses were performed within 2-4 hours of surgery and at 4 and 7 days after surgery. Celiotomy and 6% and 18% hepatectomy resulted in changes in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase concentrations that were consistent with muscle trauma. Celiotomy and 6% hepatectomy did not significantly alter the results of galactose clearance tests; however, 18% hepatectomy resulted in significant reduction in galactose clearance and galactose clearance as a function of body surface area (GEC-SA). Galactose single-point concentrations were not significantly elevated at any time during the experiments; however, single-point concentrations were strongly correlated with galactose clearance and GEC-SA values, especially at 80 minutes after galactose injection. Serum bile acid levels were not significantly elevated after celiotomy or partial hepatectomy. Measurement of galactose single-point concentrations has the potential to be a simple, sensitive method of screening for reduced hepatic function in birds. Galactose clearance and GEC-SA have the potential to be sensitive assays of hepatic functional mass, for use as a noninvasive method of monitoring hepatic function.
| Original language | English |
|---|---|
| Pages (from-to) | 164-171 |
| Number of pages | 8 |
| Journal | Journal of Avian Medicine and Surgery |
| Volume | 14 |
| Issue number | 3 |
| Publication status | Published - 01 Dec 2000 |
Fingerprint
Cite this
}
Assessment of liver function in galahs (Eolophus roseicapillus) after partial hepatectomy : A comparison of plasma enzyme concentrations, serum bile acid levels, and galactose clearance tests. / Jaensch, Susan M.; Cullen, Len; Raidal, Shane R.
In: Journal of Avian Medicine and Surgery, Vol. 14, No. 3, 01.12.2000, p. 164-171.Research output: Contribution to journal › Article
TY - JOUR
T1 - Assessment of liver function in galahs (Eolophus roseicapillus) after partial hepatectomy
T2 - A comparison of plasma enzyme concentrations, serum bile acid levels, and galactose clearance tests
AU - Jaensch, Susan M.
AU - Cullen, Len
AU - Raidal, Shane R.
PY - 2000/12/1
Y1 - 2000/12/1
N2 - The relative sensitivity of conventional assays of liver function was evaluated in comparison with galactose clearance, a test of liver function used in humans since the 1960s. Results of galactose clearance tests were compared with plasma enzyme concentrations and serum bile acid levels in clinically normal galahs (Eolophus roseicapillus; n = 8), after celiotomy (n = 4), and after 6% or 18% hepatectomy (n = 8, respectively). Clearance tests and biochemical analyses were performed within 2-4 hours of surgery and at 4 and 7 days after surgery. Celiotomy and 6% and 18% hepatectomy resulted in changes in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase concentrations that were consistent with muscle trauma. Celiotomy and 6% hepatectomy did not significantly alter the results of galactose clearance tests; however, 18% hepatectomy resulted in significant reduction in galactose clearance and galactose clearance as a function of body surface area (GEC-SA). Galactose single-point concentrations were not significantly elevated at any time during the experiments; however, single-point concentrations were strongly correlated with galactose clearance and GEC-SA values, especially at 80 minutes after galactose injection. Serum bile acid levels were not significantly elevated after celiotomy or partial hepatectomy. Measurement of galactose single-point concentrations has the potential to be a simple, sensitive method of screening for reduced hepatic function in birds. Galactose clearance and GEC-SA have the potential to be sensitive assays of hepatic functional mass, for use as a noninvasive method of monitoring hepatic function.
AB - The relative sensitivity of conventional assays of liver function was evaluated in comparison with galactose clearance, a test of liver function used in humans since the 1960s. Results of galactose clearance tests were compared with plasma enzyme concentrations and serum bile acid levels in clinically normal galahs (Eolophus roseicapillus; n = 8), after celiotomy (n = 4), and after 6% or 18% hepatectomy (n = 8, respectively). Clearance tests and biochemical analyses were performed within 2-4 hours of surgery and at 4 and 7 days after surgery. Celiotomy and 6% and 18% hepatectomy resulted in changes in alkaline phosphatase, aspartate aminotransferase, and alanine aminotransferase concentrations that were consistent with muscle trauma. Celiotomy and 6% hepatectomy did not significantly alter the results of galactose clearance tests; however, 18% hepatectomy resulted in significant reduction in galactose clearance and galactose clearance as a function of body surface area (GEC-SA). Galactose single-point concentrations were not significantly elevated at any time during the experiments; however, single-point concentrations were strongly correlated with galactose clearance and GEC-SA values, especially at 80 minutes after galactose injection. Serum bile acid levels were not significantly elevated after celiotomy or partial hepatectomy. Measurement of galactose single-point concentrations has the potential to be a simple, sensitive method of screening for reduced hepatic function in birds. Galactose clearance and GEC-SA have the potential to be sensitive assays of hepatic functional mass, for use as a noninvasive method of monitoring hepatic function.
KW - Bile acids
KW - Biopsy
KW - Birds
KW - Eolophus roseicapillus
KW - Galactose clearance
KW - Galahs
KW - Hepatectomy
KW - Hepatic
KW - Liver function tests
UR - http://www.scopus.com/inward/record.url?scp=0001228963&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0001228963&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:0001228963
VL - 14
SP - 164
EP - 171
JO - Journal of Avian Medicine and Surgery
JF - Journal of Avian Medicine and Surgery
SN - 1082-6742
IS - 3
ER -