A full length recombinant beak and feather disease virus (BFDV) capsid protein was expressed using the bacterial expression system PinPointÂ® in E. coli as a candidate antigen for immunising psittacine birds. The recombinant protein reacted with sera from naturally immune cockatoo and chickens experimentally inoculated with BFDV and the recombinant BFDV capsid protein induced an antibody response in two inoculated sheep that was detectable by Western blot and immunohistochemistry. These results confirmed that antigenic epitopes of the BFDV had been conserved in the recombinant protein and are involved in the generation of an antibody response. Haemagglutination inhibition assay demonstrated that a psittacine bird inoculated with the recombinant protein produced antibodies that inhibited the haemagglutinating activity of BDFV.
Bonne, N., Shearer, P. L., Sharp, M., Clark, P., & Raidal, S. (2009). Assessment of recombinant beak and feather disease virus (BFDV) capsid protein as a vaccine for psittacine beak and feather disease (PBFD). Journal of General Virology, 90(3), 640-647. https://doi.org/10.1099/vir.0.006932-0