Association of molecular markers with polyphenol oxidase activity in selected wheat genotypes

Zaigham Abbas, Bushra Javaid, Nadia Majeed, Ata Ur Rehman, S. M.Saqlan Naqvi

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Abstract

Wheat (Triticum aestivum L.), a major staple food for the people of Pakistan and other Asian countries, is used as bread, chapatti, porridge, noodles and many other. It is established that color quality of wheat products depend on chemical and enzymatic factors especially the polyphenol oxidases (PPOs). These are copper containing enzymes which induce browning in wheat-based products. Various procedures for determining PPO activity available and differences in PPO activity among wheat genotypes have been documented. In present study, an attempt was made to establish the association of molecular markers with polyphenol oxidase activity in wheat genotypes having very high or very low PPO activities. Twelve pairs of markers were used out of which only three primer pairs viz. PPO43, PPO30 and WP2-2 yielded specific pattern discriminating high and low PPO genotypes. Cluster analysis for all 12 markers revealed that all the low PPO lline share the same sub cluster, but high PPO lines were dispersed in different clusters.

Original languageEnglish
Pages (from-to)689-692
Number of pages4
JournalPakistan Journal of Botany
Volume48
Issue number2
Publication statusPublished - Apr 2016

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