Association of single nucleotide polymorphisms in the interleukin-12 receptor beta-2 gene with Johne's disease and production traits in dairy cattle

A Skelding, B.S. Sharma, C.P. Verschoor, SD Pant, F.S. Schenkel, H Boermans, N.A. Karrow

Research output: Other contribution to conferenceAbstract

Abstract

Mastitis and Johne’s disease are inflammatory conditions that
cause significant annual monetary losses to the dairy industry.
Interleukin-12 is a pro-inflammatory cytokine produced by
macrophages and dendritic cells as part of the host response to
infection with intracellular bacteria. Its ligand, the IL-12
receptor, is a heterodimer comprised of subunits b-1 and b-2
that are each coded by separate genes. Recent studies have
reported that increased IL-12 receptor expression is associated
with chronic inflammatory disease of the small intestine in
humans, and Staphylococcus aureus induced bovine mastitis.
We hypothesize that genetic variants in the IL-12 receptor gene
may influence susceptibility to mastitis and Johne’s disease in
dairy cattle. Based on this, single nucleotide polymorphisms
(SNPs) were identified in the bovine IL-12 b-2 receptor gene,
and five SNPs were found in total: three in the 5? upstream
region in the putative promoter of the gene, one in exon 2, and
one in the 3? untranslated region. An association study using
493 Holstein bulls and 531 dairy cows was carried out to
investigate associations among these SNPs and mastitis and
Johne’s disease, respectively. Preliminary analysis found an
association between c.-406AG and c.-511AG and Johne’s
disease (P0.004). No association was found with mastitis
using bull estimated breeding values for milk somatic cell
score; however, associations were found with the production
traits, milk yield and protein yield.
Original languageEnglish
Pages154-154
Number of pages1
Publication statusPublished - 2009
EventCanadian Society of Animal Science Annual Meeting - Montreal, Quebec, Canada
Duration: 16 Mar 200818 Mar 2008

Conference

ConferenceCanadian Society of Animal Science Annual Meeting
CountryCanada
CityMontreal, Quebec
Period16/03/0818/03/08

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    Skelding, A., Sharma, B. S., Verschoor, C. P., Pant, SD., Schenkel, F. S., Boermans, H., & Karrow, N. A. (2009). Association of single nucleotide polymorphisms in the interleukin-12 receptor beta-2 gene with Johne's disease and production traits in dairy cattle. 154-154. Abstract from Canadian Society of Animal Science Annual Meeting, Montreal, Quebec, Canada.