Atomic force microscopy on chromosomes, chromatin and DNA: A review

Wouter Kalle; Padraig Strappe

doi:10.1016/j.micron.2012.04.004

Atomic force microscopy on chromosomes, chromatin and DNA: A review

Wouter Kalle, Padraig Strappe

Research output: Contribution to journal › Article › peer-review

22 Citations (Scopus)

Abstract

The purpose of this review is to discuss the achievements and progress that has been made in the use ofatomic force microscopy in DNA related research in the last 25 years. For this review DNA related researchis split up in chromosomal-, chromatin- and DNA focused research to achieve a logical flow from large- tosmaller structures. The focus of this review is not only on the AFM as imaging tool but also on the AFM asmeasuring tool using force spectroscopy, as therein lays its greatest advantage and future. The amazingtechnological and experimental progress that has been made during the last 25 years is too extensiveto fully cover in this review but some key developments and experiments have been described to givean overview of the evolution of AFM use from 'imaging tool' to 'measurement tool' on chromosomes,chromatin and DNA.

Original language	English
Pages (from-to)	1224-1231
Number of pages	8
Journal	Micron
Volume	43
Issue number	12
DOIs	https://doi.org/10.1016/j.micron.2012.04.004
Publication status	Published - Dec 2012

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abstract = "The purpose of this review is to discuss the achievements and progress that has been made in the use ofatomic force microscopy in DNA related research in the last 25 years. For this review DNA related researchis split up in chromosomal-, chromatin- and DNA focused research to achieve a logical flow from large- tosmaller structures. The focus of this review is not only on the AFM as imaging tool but also on the AFM asmeasuring tool using force spectroscopy, as therein lays its greatest advantage and future. The amazingtechnological and experimental progress that has been made during the last 25 years is too extensiveto fully cover in this review but some key developments and experiments have been described to givean overview of the evolution of AFM use from 'imaging tool' to 'measurement tool' on chromosomes,chromatin and DNA.",

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AU - Strappe, Padraig

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KW - Chromatin

KW - Chromosome

KW - DNA

KW - In situ hybridisation

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