Non-enzymatic glycation forms advanced glycation end-products (AGEs), which may influence pathogenesis of diabetic complications and their measurement may be of great use in disease management and the prevention of complications. One way of doing this is by measuring their auto-fluorescence, but the properties of these metabolites are not clear. This study investigated the possible auto-fluorescence of collagen IV, elastin and albumin during incubation with glucose and/or fructose in vitro over a 12 week period; these investigations were duplicated separately in the presence of urea and creatinine to mimic the presence of non-protein nitrogenous (NPN) wastes. Auto-fluorescence was observed when collagen IV was incubated with glucose separately and with glucose and NPN wastes combined. No auto-fluorescence was seen when albumin or elastin were incubated with glucose or when each of the three proteins was incubated with fructose. It is possible that AGEs caused the auto-fluorescence and this may be useful in detection of diabetic complications and its management. The effects of urea and creatinine on the autofluorescence could not be clearly established therefore further research is required.
|Number of pages||6|
|Journal||Australian Journal of Medical Science|
|Publication status||Published - Feb 2009|