Automated and rapid ADAMTS13 testing using chemiluminescence: Utility for identification or exclusion of TTP and beyond

Emmanuel J Favaloro, Kent Chapman, Soma Mohammed, Ronny Vong, Leonardo Pasalic

Research output: Book chapter/Published conference paperChapter (peer-reviewed)peer-review

2 Citations (Scopus)

Abstract

Thrombotic thrombocytopenic purpura (TTP) is a prothrombotic condition caused by a significant deficiency of the enzyme, ADAMTS13 (a disintegrin and metalloproteinase with a thrombospondin type 1 motif, member 13). In the absence of adequate levels of ADAMTS13 (i.e., in TTP), plasma VWF accumulates, in particular as "ultra-large" VWF multimers, and this leads to pathological platelet aggregation and thrombosis. In addition to TTP, ADAMTS13 may be mildly to moderately reduced in a range of other conditions, including secondary thrombotic microangiopathies (TMA) such as those caused by infections (e.g., hemolytic uremic syndrome (HUS)), liver disease, disseminated intravascular coagulation (DIC), and sepsis, during acute/chronic inflammatory conditions, and sometimes also in COVID-19 (coronavirus disease 2019)). ADAMTS13 can be detected by a variety of techniques, including ELISA (enzyme-linked immunosorbent assay), FRET (fluorescence resonance energy transfer) and by chemiluminescence immunoassay (CLIA). The current report describes a protocol for assessment of ADAMTS13 by CLIA. This protocol reflects a rapid test able to be performed within 35 min on the AcuStar instrument (Werfen/Instrumentation Laboratory), although certain regional approvals may also permit this testing to be performed on a BioFlash instrument from the same manufacturer.
Original languageEnglish
Title of host publicationHemostasis and Thrombosis
Subtitle of host publicationMethods and Protocols
EditorsEmmanuel J. Favaloro , Robert C. Gosselin
Place of PublicationNew York
PublisherHumana Press
Chapter32
Pages487-504
Number of pages18
Edition2nd
ISBN (Electronic)9781071631751
ISBN (Print)9781071631744
DOIs
Publication statusPublished - 2023

Publication series

NameMethods in Molecular Biology
Volume2663
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

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