B-Cell epitope mapping using a library of overlapping synthetic peptides in an enzyme-linked immunosorbent assay

Thiru Vanniasinkam, Mary D. Barton, Tongted Phumoonna Das, Michael W. Heuzenroeder

Research output: Book chapter/Published conference paperChapter in textbook/reference bookpeer-review

1 Citation (Scopus)

Abstract

This chapter describes a strategy for mapping linear B-cell epitopes of proteins using synthetic biotinylated peptides in an ELISA. A set of overlapping peptides were designed based upon a known amino acid sequence of the target protein, VapA (Virulence-associated Protein A) of the bacterium Rhodococcus equi, an important pulmonary pathogen in foals. The peptides synthesized as biotinylated peptides were coated directly onto micro titer plates which had been pre-coated with NeutrAvidin™ and used to screen sera from foals confirmed to have R. equi disease. A linear B-cell epitope was identified which corresponded to a 20Â mer sequence of the VapA protein.
Original languageEnglish
Title of host publicationEpitope mapping protocols
EditorsJohan Rockberg, Johan Nilvebrant
Place of PublicationNew York, NY
PublisherHumana Press Inc.
Chapter8
Pages121-128
Number of pages8
Edition3rd
ISBN (Electronic)9781493978410
ISBN (Print)9781493978397
DOIs
Publication statusPublished - 01 May 2018

Publication series

NameMethods in molecular biology
Volume1785
ISSN (Print)1064-3745

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