Bee Venom Inhibits Hepatic Fibrosis Through Suppression of Pro-fibrogenic Cytokine Expression

Soo-Jung Kim, Ji-Hyun Park, Kyung-Hyun Kim, Woo-Ram Lee, Young-Chae Chang, Kwan-Kyu Park, Kwang-Gill Lee, Sang-Mi Han, Joo-Hong Yeo, Sokcheon Pak

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Bee venom (BV) has a long tradition of use for the control of pain and inflammation in various chronic diseases. Carbon tetrachloride (CCl4) is well known to induce hepatotoxicity after being metabolized to highly reactive trichloromethyl free radical and its peroxy radical. The purpose of the current study was to examine whether BV regulates the pro-inflammation and fibrosis related genes against a mouse model of hepatic fibrosis induced by CCl4 and ethanol-treated hepatocytes (ETH). Test mice were administered with CCl4 (2 ml/mg) and hepatocytes were treated with 25 mM ethanol. BV was added to the final concentration of 0.05-0.5 mg/kg and 1-100 ng/ml for in vivo and in vitro testing, respectively. Fibrotic livers and ETH were used for the measurement of hepatocyte necrosis, pro-inflammatory cytokines and fibrogenic genes. BV suppressed CCl4-induced hepatocyte necrosis markers of serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT). It also inhibited the secretion of interleukin (IL)-1β and tumor necrosis factor (TNF)-ÃŽ±. Moreover, BV inhibited CCl4-induced expression of transforming growth factor (TGF)-β1, ÃŽ±-smooth muscle actin (SMA) and fibronectin. Similarly, ETH exhibited significant supression of IL-1β, TNF-ÃŽ±, TGF-β1 and fibronectin when cultured with BV. These results suggest that BV possesses anti-fibrogenic properties that are mediated by suppression of pro-inflammatory cytokines and fibrogenic gene expression. BV has substantial therapeutic potential for the treatment of fibrotic diseases.
Original languageEnglish
Pages (from-to)921-935
Number of pages15
JournalAmerican Journal of Chinese Medicine
Issue number5
Publication statusPublished - 2010

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    Kim, S-J., Park, J-H., Kim, K-H., Lee, W-R., Chang, Y-C., Park, K-K., Lee, K-G., Han, S-M., Yeo, J-H., & Pak, S. (2010). Bee Venom Inhibits Hepatic Fibrosis Through Suppression of Pro-fibrogenic Cytokine Expression. American Journal of Chinese Medicine, 38(5), 921-935.