Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse

Woon-Hae Kim; Hyun-Jin An; Jung-Yeon Kim; Mi-Gyeong Gwon; Hyemin Gu; Minji Jeon; Woo Jung Sung; Sang-Mi Han; Sokcheon Pak; Min-Kyung Kim; Kwan-Kyu Park

doi:10.1038/s41598-017-17873-2

Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse

Woon-Hae Kim, Hyun-Jin An, Jung-Yeon Kim, Mi-Gyeong Gwon, Hyemin Gu, Minji Jeon, Woo Jung Sung, Sang-Mi Han, Sokcheon Pak, Min-Kyung Kim, Kwan-Kyu Park

Dentistry and Medical Sciences

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72 Downloads (Pure)

Abstract

Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored. Therefore, we investigated the anti-allergic effects of melittin. AD was induced by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were extracted from the sacrificed mice were used to demonstrate the effects of melittin through various molecular biological methods. The results showed that OVA-induced skin thickening and inflammatory infiltration were decreased in the melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and imbalanced inflammatory mediators. Furthermore, melittin inhibited IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 activation in human keratinocytes. In summary, this study has shown that melittin ameliorated OVA-induced AD-like symptoms from various perspectives. The findings of this study may be the first evidence of the anti-inflammatory effects of melittin on OVA-induced AD.

Original language	English
Article number	17679
Pages (from-to)	1-12
Number of pages	12
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-17873-2
Publication status	Published - Dec 2017

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19921974_Published article-OAFinal published version, 5.59 MBLicence: CC BY-NC-ND

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title = "Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse",

abstract = "Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored. Therefore, we investigated the anti-allergic effects of melittin. AD was induced by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were extracted from the sacrificed mice were used to demonstrate the effects of melittin through various molecular biological methods. The results showed that OVA-induced skin thickening and inflammatory infiltration were decreased in the melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and imbalanced inflammatory mediators. Furthermore, melittin inhibited IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 activation in human keratinocytes. In summary, this study has shown that melittin ameliorated OVA-induced AD-like symptoms from various perspectives. The findings of this study may be the first evidence of the anti-inflammatory effects of melittin on OVA-induced AD.",

author = "Woon-Hae Kim and Hyun-Jin An and Jung-Yeon Kim and Mi-Gyeong Gwon and Hyemin Gu and Minji Jeon and Sung, {Woo Jung} and Sang-Mi Han and Sokcheon Pak and Min-Kyung Kim and Kwan-Kyu Park",

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year = "2017",

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doi = "10.1038/s41598-017-17873-2",

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journal = "Scientific Reports",

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T1 - Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse

AU - Kim, Woon-Hae

AU - An, Hyun-Jin

AU - Kim, Jung-Yeon

AU - Gwon, Mi-Gyeong

AU - Gu, Hyemin

AU - Jeon, Minji

AU - Sung, Woo Jung

AU - Han, Sang-Mi

AU - Pak, Sokcheon

AU - Kim, Min-Kyung

AU - Park, Kwan-Kyu

N1 - Includes bibliographical references.

PY - 2017/12

Y1 - 2017/12

N2 - Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored. Therefore, we investigated the anti-allergic effects of melittin. AD was induced by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were extracted from the sacrificed mice were used to demonstrate the effects of melittin through various molecular biological methods. The results showed that OVA-induced skin thickening and inflammatory infiltration were decreased in the melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and imbalanced inflammatory mediators. Furthermore, melittin inhibited IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 activation in human keratinocytes. In summary, this study has shown that melittin ameliorated OVA-induced AD-like symptoms from various perspectives. The findings of this study may be the first evidence of the anti-inflammatory effects of melittin on OVA-induced AD.

AB - Atopic dermatitis (AD) is an inflammatory skin disease characterized by intense pruritus and relapsable eczematous lesions. The hallmarks of AD are defects in the epidermal barrier and immunoglobulin E (IgE)-mediated sensitization to several environmental allergens, as well as an immune disorder mediated by an imbalance toward T-helper-2 response. Melittin, a major component of bee venom, has been studied in various inflammatory diseases. However, the beneficial effects of melittin on mouse with AD-like symptoms have not been explored. Therefore, we investigated the anti-allergic effects of melittin. AD was induced by ovalbumin (OVA) patch. After agent treatment, skin tissues and sera were extracted from the sacrificed mice were used to demonstrate the effects of melittin through various molecular biological methods. The results showed that OVA-induced skin thickening and inflammatory infiltration were decreased in the melittin-treated group. Melittin prevented OVA-induced filaggrin deficiency and imbalanced inflammatory mediators. Furthermore, melittin inhibited IL-4/IL-13-induced filaggrin downregulation through the blockade of STAT3 activation in human keratinocytes. In summary, this study has shown that melittin ameliorated OVA-induced AD-like symptoms from various perspectives. The findings of this study may be the first evidence of the anti-inflammatory effects of melittin on OVA-induced AD.

U2 - 10.1038/s41598-017-17873-2

DO - 10.1038/s41598-017-17873-2

M3 - Article

C2 - 29247241

SN - 2045-2322

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SP - 1

EP - 12

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 17679

ER -

Beneficial effects of melittin on ovalbumin-induced atopic dermatitis in mouse

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