Biological control of Cylindrocarpon spp. on grapevine roots.

Melanie Weckert

Biological control of Cylindrocarpon spp. on grapevine roots.

Melanie Weckert

National Wine and Grape Industry Centre

Research output: Book chapter/Published conference paper › Conference paper

26 Downloads (Pure)

Abstract

Those inoculated with Streptomyces sp. or with C. macrodidymum and Streptomyces sp. together, were healthy. SEM showed evidence of parasitism of C. macrodidymum conidia by Streptomyces sp.. Table 1: Potted Chardonnay vines 14 weeks after inoculation1. Root length (cm) Root rot 2Control 333 a 1.0 aC. macrodidymum 28 b 4.0 bC. macrodidymum plus Streptomyces sp. 258 a 1.0 aStreptomyces sp. 305 a 0.8 a1 ANOVA performed for data from 4 replicate pots; 2 0 = no disease symptoms; 1 = 1-25%; 2 = 26-50%; 3 = 51-75%; 4 = 76-100% root rot. Values within a column followed by the same letter are not significantly different based on l.s.d. (P = 0.05). Discussion:Streptomyces sp. was shown to be antagonistic towards Cylindrocarpon macrodidymum. Future work will further investigate parasitism and induction of plant degenes by the actinomycete.for 5th ASDS Melanie Weckert, National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW12/11/08 (282 words)Biological control of Cylindrocarpon spp. on grapevine rootsStreptomyces sp. isolated from vineyard soil in Wagga Wagga, NSW, decreased vegetative growth of Cylindrocarpon liriodendri and Cylindrocarpon macrodidymum on potato dextrose agar. The actinomycete also demonstrated biocontrol activity against C. liriodendri in a long term Vitis vinifera cv. Chardonnay soil pot experiment. In order to study the interactions between the actinomycete and pathogenic fungus, a gnotobiotic pot experiment was conducted with Vitis vinifera var. Chardonnay rooted cuttings in sterile sand/peat. Materials and Methods Two-month old Chardonnay rooted cuttings were inoculated with Streptomyces sp. and then with Cylindrocarpon macrodidymum three weeks later. Four replicate uninoculated vines plus vines inoculated with C. macrodidymum alone; C. macrodidymum and Streptomyces sp. together; and Streptomyces alone were grown in enclosed pots in a glasshouse for 14 weeks. Results:The vines inoculated solely with C. macrodidymum were dead after 14 weeks.

Original language	English
Title of host publication	Fifth Australasian Soilborne Diseases Symposium
Editors	Gavin Ash
Place of Publication	Kingston, ACT
Publisher	Conference Logistics
Pages	96-97
Number of pages	2
Volume	38
Publication status	Published - 2009
Event	Australasian Plant Pathology Association Conference - Thredbo, NSW, Australia Duration: 05 Feb 2009 → 07 Feb 2009

Conference

Conference	Australasian Plant Pathology Association Conference
Country	Australia
Period	05/02/09 → 07/02/09

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20769 conference paperAccepted author manuscript, 136 KB

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Weckert, M. (2009). Biological control of Cylindrocarpon spp. on grapevine roots. In G. Ash (Ed.), Fifth Australasian Soilborne Diseases Symposium (Vol. 38, pp. 96-97). Conference Logistics.

@inproceedings{e81003b87978475cae752357b4370886,

title = "Biological control of Cylindrocarpon spp. on grapevine roots.",

abstract = "Those inoculated with Streptomyces sp. or with C. macrodidymum and Streptomyces sp. together, were healthy. SEM showed evidence of parasitism of C. macrodidymum conidia by Streptomyces sp.. Table 1: Potted Chardonnay vines 14 weeks after inoculation1. Root length (cm) Root rot 2Control 333 a 1.0 aC. macrodidymum 28 b 4.0 bC. macrodidymum plus Streptomyces sp. 258 a 1.0 aStreptomyces sp. 305 a 0.8 a1 ANOVA performed for data from 4 replicate pots; 2 0 = no disease symptoms; 1 = 1-25%; 2 = 26-50%; 3 = 51-75%; 4 = 76-100% root rot. Values within a column followed by the same letter are not significantly different based on l.s.d. (P = 0.05). Discussion:Streptomyces sp. was shown to be antagonistic towards Cylindrocarpon macrodidymum. Future work will further investigate parasitism and induction of plant degenes by the actinomycete.for 5th ASDS Melanie Weckert, National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW12/11/08 (282 words)Biological control of Cylindrocarpon spp. on grapevine rootsStreptomyces sp. isolated from vineyard soil in Wagga Wagga, NSW, decreased vegetative growth of Cylindrocarpon liriodendri and Cylindrocarpon macrodidymum on potato dextrose agar. The actinomycete also demonstrated biocontrol activity against C. liriodendri in a long term Vitis vinifera cv. Chardonnay soil pot experiment. In order to study the interactions between the actinomycete and pathogenic fungus, a gnotobiotic pot experiment was conducted with Vitis vinifera var. Chardonnay rooted cuttings in sterile sand/peat. Materials and Methods Two-month old Chardonnay rooted cuttings were inoculated with Streptomyces sp. and then with Cylindrocarpon macrodidymum three weeks later. Four replicate uninoculated vines plus vines inoculated with C. macrodidymum alone; C. macrodidymum and Streptomyces sp. together; and Streptomyces alone were grown in enclosed pots in a glasshouse for 14 weeks. Results:The vines inoculated solely with C. macrodidymum were dead after 14 weeks.",

keywords = "Open access version available, Cylindrocarpon liriodendri, Cylindrocarpon macrodidymum, Streptomyces",

author = "Melanie Weckert",

note = "Imported on 03 May 2017 - DigiTool details were: 086 FoR could not be migrated (270403 - ). publisher = Kingston, ACT: Conference Logistics, 2009. editor/s (773b) = Dr Gavin Ash; Event dates (773o) = 5-7 February, 2009; Parent title (773t) = Australasian Plant Pathology Association Conference.; Australasian Plant Pathology Association Conference ; Conference date: 05-02-2009 Through 07-02-2009",

year = "2009",

language = "English",

volume = "38",

pages = "96--97",

editor = "Gavin Ash",

booktitle = "Fifth Australasian Soilborne Diseases Symposium",

publisher = "Conference Logistics",

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TY - GEN

T1 - Biological control of Cylindrocarpon spp. on grapevine roots.

AU - Weckert, Melanie

N1 - Imported on 03 May 2017 - DigiTool details were: 086 FoR could not be migrated (270403 - ). publisher = Kingston, ACT: Conference Logistics, 2009. editor/s (773b) = Dr Gavin Ash; Event dates (773o) = 5-7 February, 2009; Parent title (773t) = Australasian Plant Pathology Association Conference.

PY - 2009

Y1 - 2009

N2 - Those inoculated with Streptomyces sp. or with C. macrodidymum and Streptomyces sp. together, were healthy. SEM showed evidence of parasitism of C. macrodidymum conidia by Streptomyces sp.. Table 1: Potted Chardonnay vines 14 weeks after inoculation1. Root length (cm) Root rot 2Control 333 a 1.0 aC. macrodidymum 28 b 4.0 bC. macrodidymum plus Streptomyces sp. 258 a 1.0 aStreptomyces sp. 305 a 0.8 a1 ANOVA performed for data from 4 replicate pots; 2 0 = no disease symptoms; 1 = 1-25%; 2 = 26-50%; 3 = 51-75%; 4 = 76-100% root rot. Values within a column followed by the same letter are not significantly different based on l.s.d. (P = 0.05). Discussion:Streptomyces sp. was shown to be antagonistic towards Cylindrocarpon macrodidymum. Future work will further investigate parasitism and induction of plant degenes by the actinomycete.for 5th ASDS Melanie Weckert, National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW12/11/08 (282 words)Biological control of Cylindrocarpon spp. on grapevine rootsStreptomyces sp. isolated from vineyard soil in Wagga Wagga, NSW, decreased vegetative growth of Cylindrocarpon liriodendri and Cylindrocarpon macrodidymum on potato dextrose agar. The actinomycete also demonstrated biocontrol activity against C. liriodendri in a long term Vitis vinifera cv. Chardonnay soil pot experiment. In order to study the interactions between the actinomycete and pathogenic fungus, a gnotobiotic pot experiment was conducted with Vitis vinifera var. Chardonnay rooted cuttings in sterile sand/peat. Materials and Methods Two-month old Chardonnay rooted cuttings were inoculated with Streptomyces sp. and then with Cylindrocarpon macrodidymum three weeks later. Four replicate uninoculated vines plus vines inoculated with C. macrodidymum alone; C. macrodidymum and Streptomyces sp. together; and Streptomyces alone were grown in enclosed pots in a glasshouse for 14 weeks. Results:The vines inoculated solely with C. macrodidymum were dead after 14 weeks.

AB - Those inoculated with Streptomyces sp. or with C. macrodidymum and Streptomyces sp. together, were healthy. SEM showed evidence of parasitism of C. macrodidymum conidia by Streptomyces sp.. Table 1: Potted Chardonnay vines 14 weeks after inoculation1. Root length (cm) Root rot 2Control 333 a 1.0 aC. macrodidymum 28 b 4.0 bC. macrodidymum plus Streptomyces sp. 258 a 1.0 aStreptomyces sp. 305 a 0.8 a1 ANOVA performed for data from 4 replicate pots; 2 0 = no disease symptoms; 1 = 1-25%; 2 = 26-50%; 3 = 51-75%; 4 = 76-100% root rot. Values within a column followed by the same letter are not significantly different based on l.s.d. (P = 0.05). Discussion:Streptomyces sp. was shown to be antagonistic towards Cylindrocarpon macrodidymum. Future work will further investigate parasitism and induction of plant degenes by the actinomycete.for 5th ASDS Melanie Weckert, National Wine and Grape Industry Centre, Charles Sturt University, Wagga Wagga, NSW12/11/08 (282 words)Biological control of Cylindrocarpon spp. on grapevine rootsStreptomyces sp. isolated from vineyard soil in Wagga Wagga, NSW, decreased vegetative growth of Cylindrocarpon liriodendri and Cylindrocarpon macrodidymum on potato dextrose agar. The actinomycete also demonstrated biocontrol activity against C. liriodendri in a long term Vitis vinifera cv. Chardonnay soil pot experiment. In order to study the interactions between the actinomycete and pathogenic fungus, a gnotobiotic pot experiment was conducted with Vitis vinifera var. Chardonnay rooted cuttings in sterile sand/peat. Materials and Methods Two-month old Chardonnay rooted cuttings were inoculated with Streptomyces sp. and then with Cylindrocarpon macrodidymum three weeks later. Four replicate uninoculated vines plus vines inoculated with C. macrodidymum alone; C. macrodidymum and Streptomyces sp. together; and Streptomyces alone were grown in enclosed pots in a glasshouse for 14 weeks. Results:The vines inoculated solely with C. macrodidymum were dead after 14 weeks.

KW - Open access version available

KW - Cylindrocarpon liriodendri

KW - Cylindrocarpon macrodidymum

KW - Streptomyces

M3 - Conference paper

VL - 38

SP - 96

EP - 97

BT - Fifth Australasian Soilborne Diseases Symposium

A2 - Ash, Gavin

PB - Conference Logistics

CY - Kingston, ACT

T2 - Australasian Plant Pathology Association Conference

Y2 - 5 February 2009 through 7 February 2009

ER -