TY - JOUR
T1 - Bioprospecting traditional Pakistani medicinal plants for potent antioxidants
AU - Inayatullah, Samia
AU - Prenzler, Paul
AU - Obied, Hassan
AU - Rehman, Ata-ur
AU - Mirza, Bushra
N1 - Imported on 12 Apr 2017 - DigiTool details were: month (773h) = May, 2012; Journal title (773t) = Food Chemistry. ISSNs: 0308-8146;
PY - 2012/5
Y1 - 2012/5
N2 - Antioxidant potential of four methanol extracts from three selected plant species, namely Salvia nubicola(Lamiaceae), Acer oblongifolium (Aceraceae) and Hedera nepalensis (Araliaceae) was measured usingassays in aqueous and lipid systems. Antioxidant activities were investigated in aqueous systems byusing DPPH radical-scavenging assay, ABTS radical-scavenging assay and DNA protection assay, whileantioxidant activity in a lipid system was determined by using the thiobarbituric acid-reactive substances(TBARS) assay. Additionally, the Folin-Ciocalteu method was used to measure total phenolic content.Methanol extracts of leaves and flowers of S. nubicola showed the highest Trolox equivalent (TE) valuesin the case of the DPPH -- CORRECTION REQUIRED HERE -- 2; assay, 2484 ± 4.9 mmol TE/g extract, as well as total phenolic content,139 ± 0.2 mg gallic acid equivalents/g extract. Three fractions (A'C) of the methanol extract of S. nubicolaleaves and flowers were produced by semi-preparative HPLC. Fraction B was found to be the most activein the DPPH radical-scavenging assay and had the highest total phenol content. HPLC-DAD and LC'MSrevealed rosmarinic acid in S. nubicola extracts and chlorogenic acid and rutin in H. nepalensis extractsas the main phenolic antioxidants.
AB - Antioxidant potential of four methanol extracts from three selected plant species, namely Salvia nubicola(Lamiaceae), Acer oblongifolium (Aceraceae) and Hedera nepalensis (Araliaceae) was measured usingassays in aqueous and lipid systems. Antioxidant activities were investigated in aqueous systems byusing DPPH radical-scavenging assay, ABTS radical-scavenging assay and DNA protection assay, whileantioxidant activity in a lipid system was determined by using the thiobarbituric acid-reactive substances(TBARS) assay. Additionally, the Folin-Ciocalteu method was used to measure total phenolic content.Methanol extracts of leaves and flowers of S. nubicola showed the highest Trolox equivalent (TE) valuesin the case of the DPPH -- CORRECTION REQUIRED HERE -- 2; assay, 2484 ± 4.9 mmol TE/g extract, as well as total phenolic content,139 ± 0.2 mg gallic acid equivalents/g extract. Three fractions (A'C) of the methanol extract of S. nubicolaleaves and flowers were produced by semi-preparative HPLC. Fraction B was found to be the most activein the DPPH radical-scavenging assay and had the highest total phenol content. HPLC-DAD and LC'MSrevealed rosmarinic acid in S. nubicola extracts and chlorogenic acid and rutin in H. nepalensis extractsas the main phenolic antioxidants.
KW - Open access version available
KW - Acer oblongifolium (Aceraceae)
KW - Antioxidant assays
KW - Bioactivity-guided fractionation
KW - Folin-Ciocalteau
KW - Hedera nepalensis (Araliaceae)
KW - Salvia nubicola (Lamiaceae)
U2 - 10.1016/j.foodchem.2011.10.060
DO - 10.1016/j.foodchem.2011.10.060
M3 - Article
SN - 0308-8146
VL - 132
SP - 222
EP - 229
JO - Food Chemistry
JF - Food Chemistry
IS - 1
ER -