Bioprospecting traditional Pakistani medicinal plants for potent antioxidants

Samia Inayatullah, Paul Prenzler, Hassan Obied, Ata-ur Rehman, Bushra Mirza

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)
40 Downloads (Pure)

Abstract

Antioxidant potential of four methanol extracts from three selected plant species, namely Salvia nubicola(Lamiaceae), Acer oblongifolium (Aceraceae) and Hedera nepalensis (Araliaceae) was measured usingassays in aqueous and lipid systems. Antioxidant activities were investigated in aqueous systems byusing DPPH radical-scavenging assay, ABTS radical-scavenging assay and DNA protection assay, whileantioxidant activity in a lipid system was determined by using the thiobarbituric acid-reactive substances(TBARS) assay. Additionally, the Folin-Ciocalteu method was used to measure total phenolic content.Methanol extracts of leaves and flowers of S. nubicola showed the highest Trolox equivalent (TE) valuesin the case of the DPPH -- CORRECTION REQUIRED HERE -- 2; assay, 2484 ± 4.9 mmol TE/g extract, as well as total phenolic content,139 ± 0.2 mg gallic acid equivalents/g extract. Three fractions (A'C) of the methanol extract of S. nubicolaleaves and flowers were produced by semi-preparative HPLC. Fraction B was found to be the most activein the DPPH radical-scavenging assay and had the highest total phenol content. HPLC-DAD and LC'MSrevealed rosmarinic acid in S. nubicola extracts and chlorogenic acid and rutin in H. nepalensis extractsas the main phenolic antioxidants.
Original languageEnglish
Pages (from-to)222-229
Number of pages8
JournalFood Chemistry
Volume132
Issue number1
DOIs
Publication statusPublished - May 2012

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