Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs

Karina Obelitz-Ryom, Stine Brandt Bering, Silja Hvid Overgaard, SImon Fristed Eskildsen, Steffen Ringgaard, Jonas Lynge Olesen, Kerstin Skovgaard, Stanislava Pankratova, Bing Wang, Anders Brunse, Anne Birgitte Heckmann, Martin Peter Rydal, Per Torp Sangild, Thomas Thymann

Research output: Contribution to journalArticle

Abstract

Optimal nutrition is important after preterm birth to facilitate normal brain development. Human milk is rich in sialic acid and preterm infants may benefit from supplementing formula with sialyllactose to support neurodevelopment. Using pigs as models, we hypothesized that sialyllactose supplementation improves brain development after preterm birth. Pigs (of either sex) were delivered by cesarean section at 90% gestation and fed a milk diet supplemented with either an oligosaccharide-enriched whey with sialyllactose (n = 20) or lactose (n = 20) for 19 days. Cognitive performance was tested in a spatial T-maze. Brains were collected for ex vivo magnetic resonance imaging (MRI), gene expression, and sialic acid measurements. For reference, term piglets (n = 14) were artificially reared under identical conditions and compared with vaginally born piglets naturally reared by the sow (n = 12). A higher proportion of sialyllactose supplemented preterm pigs reached the T-maze learning criteria relative to control preterm pigs (p < 0.05), and approximated the cognition level of term reference pigs (p < 0.01). Furthermore, supplemented pigs had upregulated genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in hippocampus. Sialyllactose supplementation did not lead to higher levels of sialic acid in the hippocampus or change MRI endpoints. Contrary, these parameters were strongly influenced by postconceptional age and postnatal rearing conditions. In conclusion, oligosaccharide-enriched whey with sialyllactose improved spatial cognition, with effects on hippocampal genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in preterm pigs. Dietary sialic acid enrichment may improve brain development in infants.
Original languageEnglish
Article number1335
Pages (from-to)1-20
Number of pages20
JournalNutrients
Volume11
Issue number6
DOIs
Publication statusPublished - 14 Jun 2019

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sialic acids
Oligosaccharides
cognition
oligosaccharides
Cognition
N-Acetylneuraminic Acid
Milk
Swine
milk
swine
myelination
brain
premature birth
gangliosides
Gangliosides
Premature Birth
Brain
hippocampus
whey
magnetic resonance imaging

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Obelitz-Ryom, K., Bering, S. B., Overgaard, S. H., Eskildsen, SI. F., Ringgaard, S., Olesen, J. L., ... Thymann, T. (2019). Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs. Nutrients, 11(6), 1-20. [1335]. https://doi.org/10.3390/nu11061335
Obelitz-Ryom, Karina ; Bering, Stine Brandt ; Overgaard, Silja Hvid ; Eskildsen, SImon Fristed ; Ringgaard, Steffen ; Olesen, Jonas Lynge ; Skovgaard, Kerstin ; Pankratova, Stanislava ; Wang, Bing ; Brunse, Anders ; Heckmann, Anne Birgitte ; Rydal, Martin Peter ; Sangild, Per Torp ; Thymann, Thomas. / Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs. In: Nutrients. 2019 ; Vol. 11, No. 6. pp. 1-20.
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abstract = "Optimal nutrition is important after preterm birth to facilitate normal brain development. Human milk is rich in sialic acid and preterm infants may benefit from supplementing formula with sialyllactose to support neurodevelopment. Using pigs as models, we hypothesized that sialyllactose supplementation improves brain development after preterm birth. Pigs (of either sex) were delivered by cesarean section at 90{\%} gestation and fed a milk diet supplemented with either an oligosaccharide-enriched whey with sialyllactose (n = 20) or lactose (n = 20) for 19 days. Cognitive performance was tested in a spatial T-maze. Brains were collected for ex vivo magnetic resonance imaging (MRI), gene expression, and sialic acid measurements. For reference, term piglets (n = 14) were artificially reared under identical conditions and compared with vaginally born piglets naturally reared by the sow (n = 12). A higher proportion of sialyllactose supplemented preterm pigs reached the T-maze learning criteria relative to control preterm pigs (p < 0.05), and approximated the cognition level of term reference pigs (p < 0.01). Furthermore, supplemented pigs had upregulated genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in hippocampus. Sialyllactose supplementation did not lead to higher levels of sialic acid in the hippocampus or change MRI endpoints. Contrary, these parameters were strongly influenced by postconceptional age and postnatal rearing conditions. In conclusion, oligosaccharide-enriched whey with sialyllactose improved spatial cognition, with effects on hippocampal genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in preterm pigs. Dietary sialic acid enrichment may improve brain development in infants.",
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Obelitz-Ryom, K, Bering, SB, Overgaard, SH, Eskildsen, SIF, Ringgaard, S, Olesen, JL, Skovgaard, K, Pankratova, S, Wang, B, Brunse, A, Heckmann, AB, Rydal, MP, Sangild, PT & Thymann, T 2019, 'Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs', Nutrients, vol. 11, no. 6, 1335, pp. 1-20. https://doi.org/10.3390/nu11061335

Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs. / Obelitz-Ryom, Karina; Bering, Stine Brandt; Overgaard, Silja Hvid; Eskildsen, SImon Fristed; Ringgaard, Steffen; Olesen, Jonas Lynge; Skovgaard, Kerstin; Pankratova, Stanislava; Wang, Bing; Brunse, Anders; Heckmann, Anne Birgitte; Rydal, Martin Peter; Sangild, Per Torp; Thymann, Thomas.

In: Nutrients, Vol. 11, No. 6, 1335, 14.06.2019, p. 1-20.

Research output: Contribution to journalArticle

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AU - Bering, Stine Brandt

AU - Overgaard, Silja Hvid

AU - Eskildsen, SImon Fristed

AU - Ringgaard, Steffen

AU - Olesen, Jonas Lynge

AU - Skovgaard, Kerstin

AU - Pankratova, Stanislava

AU - Wang, Bing

AU - Brunse, Anders

AU - Heckmann, Anne Birgitte

AU - Rydal, Martin Peter

AU - Sangild, Per Torp

AU - Thymann, Thomas

PY - 2019/6/14

Y1 - 2019/6/14

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AB - Optimal nutrition is important after preterm birth to facilitate normal brain development. Human milk is rich in sialic acid and preterm infants may benefit from supplementing formula with sialyllactose to support neurodevelopment. Using pigs as models, we hypothesized that sialyllactose supplementation improves brain development after preterm birth. Pigs (of either sex) were delivered by cesarean section at 90% gestation and fed a milk diet supplemented with either an oligosaccharide-enriched whey with sialyllactose (n = 20) or lactose (n = 20) for 19 days. Cognitive performance was tested in a spatial T-maze. Brains were collected for ex vivo magnetic resonance imaging (MRI), gene expression, and sialic acid measurements. For reference, term piglets (n = 14) were artificially reared under identical conditions and compared with vaginally born piglets naturally reared by the sow (n = 12). A higher proportion of sialyllactose supplemented preterm pigs reached the T-maze learning criteria relative to control preterm pigs (p < 0.05), and approximated the cognition level of term reference pigs (p < 0.01). Furthermore, supplemented pigs had upregulated genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in hippocampus. Sialyllactose supplementation did not lead to higher levels of sialic acid in the hippocampus or change MRI endpoints. Contrary, these parameters were strongly influenced by postconceptional age and postnatal rearing conditions. In conclusion, oligosaccharide-enriched whey with sialyllactose improved spatial cognition, with effects on hippocampal genes related to sialic acid metabolism, myelination, and ganglioside biosynthesis in preterm pigs. Dietary sialic acid enrichment may improve brain development in infants.

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Obelitz-Ryom K, Bering SB, Overgaard SH, Eskildsen SIF, Ringgaard S, Olesen JL et al. Bovine milk oligosaccharides with sialyllactose improves cognition in preterm pigs. Nutrients. 2019 Jun 14;11(6):1-20. 1335. https://doi.org/10.3390/nu11061335