Bromoperoxidase producing bacillus spp. isolated from the hypobranchial glands of a muricid mollusc are capable of tyrian purple precursor biogenesis

Ajit Kumar Ngangbam, Peter Mouatt, Joshua Smith, Daniel L.E. Waters, Kirsten Benkendorff

Research output: Contribution to journalArticle

Abstract

The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, Dicathais orbita, has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography-mass spectrometry (LC-MS). In total, 32 bacterial isolates were cultured from D. orbita hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 Vibrio spp., two Bacillus spp., one Phaeobacter sp., one Shewanella sp., one Halobacillus sp. and one Pseudoalteromonas sp. The two Bacillus species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC-MS analysis of the supernatant and cell pellets from the bromoperoxidase producing Bacillus spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic Bacillus spp. in the biosynthesis of Tyrian purple.

Original languageEnglish
Article number264
JournalMarine Drugs
Volume17
Issue number5
DOIs
Publication statusPublished - 01 Jan 2019

Fingerprint

Mollusca
Bacillus
Agar
Liquid Chromatography
Sulfates
Halobacillus
Mass Spectrometry
Pseudoalteromonas
Shewanella
Shellfish
Thiosulfates
Vibrio
Bile Acids and Salts
Citric Acid
Sucrose
bromide peroxidase
6,6'-dibromoindigo
Coloring Agents
Bacteria
Enzymes

Cite this

@article{837654f7400a42d7a21ed41567801577,
title = "Bromoperoxidase producing bacillus spp. isolated from the hypobranchial glands of a muricid mollusc are capable of tyrian purple precursor biogenesis",
abstract = "The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, Dicathais orbita, has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography-mass spectrometry (LC-MS). In total, 32 bacterial isolates were cultured from D. orbita hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 Vibrio spp., two Bacillus spp., one Phaeobacter sp., one Shewanella sp., one Halobacillus sp. and one Pseudoalteromonas sp. The two Bacillus species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC-MS analysis of the supernatant and cell pellets from the bromoperoxidase producing Bacillus spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic Bacillus spp. in the biosynthesis of Tyrian purple.",
keywords = "Bacillus, Bromoperoxidase, Shellfish purple, Tyrindoxyl sulphate, Whelk",
author = "Ngangbam, {Ajit Kumar} and Peter Mouatt and Joshua Smith and Waters, {Daniel L.E.} and Kirsten Benkendorff",
year = "2019",
month = "1",
day = "1",
doi = "10.3390/md17050264",
language = "English",
volume = "17",
journal = "Marine Drugs",
issn = "1660-3397",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "5",

}

Bromoperoxidase producing bacillus spp. isolated from the hypobranchial glands of a muricid mollusc are capable of tyrian purple precursor biogenesis. / Ngangbam, Ajit Kumar; Mouatt, Peter; Smith, Joshua; Waters, Daniel L.E.; Benkendorff, Kirsten.

In: Marine Drugs, Vol. 17, No. 5, 264, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bromoperoxidase producing bacillus spp. isolated from the hypobranchial glands of a muricid mollusc are capable of tyrian purple precursor biogenesis

AU - Ngangbam, Ajit Kumar

AU - Mouatt, Peter

AU - Smith, Joshua

AU - Waters, Daniel L.E.

AU - Benkendorff, Kirsten

PY - 2019/1/1

Y1 - 2019/1/1

N2 - The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, Dicathais orbita, has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography-mass spectrometry (LC-MS). In total, 32 bacterial isolates were cultured from D. orbita hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 Vibrio spp., two Bacillus spp., one Phaeobacter sp., one Shewanella sp., one Halobacillus sp. and one Pseudoalteromonas sp. The two Bacillus species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC-MS analysis of the supernatant and cell pellets from the bromoperoxidase producing Bacillus spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic Bacillus spp. in the biosynthesis of Tyrian purple.

AB - The secondary metabolite Tyrian purple, also known as shellfish purple and royal purple, is a dye with historical importance for humans. The biosynthetic origin of Tyrian purple in Muricidae molluscs is not currently known. A possible role for symbiotic bacteria in the production of tyrindoxyl sulphate, the precursor to Tyrian purple stored in the Australian species, Dicathais orbita, has been proposed. This study aimed to culture bacterial symbionts from the purple producing hypobranchial gland, and screen the isolates for bromoperoxidase genes using molecular methods. The ability of bromoperoxidase positive isolates to produce the brominated indole precursor to Tyrian purple was then established by extraction of the culture, and analysis by liquid chromatography-mass spectrometry (LC-MS). In total, 32 bacterial isolates were cultured from D. orbita hypobranchial glands, using marine agar, marine agar with hypobranchial gland aqueous extracts, blood agar, thiosulphate citrate bile salts sucrose agar, and cetrimide agar at pH 7.2. These included 26 Vibrio spp., two Bacillus spp., one Phaeobacter sp., one Shewanella sp., one Halobacillus sp. and one Pseudoalteromonas sp. The two Bacillus species were the only isolates found to have coding sequences for bromoperoxidase enzymes. LC-MS analysis of the supernatant and cell pellets from the bromoperoxidase producing Bacillus spp. cultured in tryptone broth, supplemented with KBr, confirmed their ability to produce the brominated precursor to Tyrian purple, tyrindoxyl sulphate. This study supports a potential role for symbiotic Bacillus spp. in the biosynthesis of Tyrian purple.

KW - Bacillus

KW - Bromoperoxidase

KW - Shellfish purple

KW - Tyrindoxyl sulphate

KW - Whelk

UR - http://www.scopus.com/inward/record.url?scp=85065639115&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85065639115&partnerID=8YFLogxK

U2 - 10.3390/md17050264

DO - 10.3390/md17050264

M3 - Article

VL - 17

JO - Marine Drugs

JF - Marine Drugs

SN - 1660-3397

IS - 5

M1 - 264

ER -