Canola proteins for human consumption: Extraction, profile and functional properties, a review

Siong Tan, Rodney Mailer, Christopher Blanchard, Samson Agboola

Research output: Contribution to journalArticlepeer-review

126 Citations (Scopus)


Canola protein isolate has been suggested as an alternative to other proteins for human food use due to a balanced amino acid profile and potential functional properties such as emulsifying, foaming and gelling abilities. This is, therefore, a review of the utilization of canola protein in human food, comprising the extraction processes for protein isolates and fractions, the molecular character of the extracted proteins, as well as their food functional properties. Majority of studies were based on protein extracted from the meal using alkaline solution, presumably due to its high nitrogen yield, followed by those utilising salt extraction combined with ultrafiltration. Characteristics of canola and its predecessor rapeseed protein fractions such as nitrogen yield, molecular weight profile, isoelectric point, solubility and thermal properties have been reported, and were found to be largely related to the extraction methods. However, very little research has been carried out on the hydrophobicity and structure profiles of the protein extracts which are highly relevant to a proper understanding of food functional properties. Alkaline extracts were generally not very suitable as functional ingredients and contradictory results about many of the measured properties of canola proteins, especially their emulsification tendencies, have also been documented. Further research into improved extraction methods is recommended, as is a more systematic approach to the measurement of desired food functional properties for valid comparison between studies.
Original languageEnglish
Pages (from-to)R16-R28
Number of pages13
JournalJournal of Food Science
Issue number1
Publication statusPublished - 2011

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