Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload

Zhi-Hua Wang, Jian Hao Pan, Xian Peng Ma, Xiao Yun Xu, Wen Hui Yu, Wen Juan Fu, Jian Ke, Chang Qiong Bi, Wei Wei, Qu Zhao, Feng-Yun Wang, Dan Tang, Kaihe Ye, Zhixian Yi, Hong Nie

Research output: Contribution to journalArticle

Abstract

Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose Injection (SGI) in rat acute myocardial infarction (AMI). Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’ consecutive intravenous administration of SGI, serum samples were used to conduct biochemical analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology and qPCR studies. Intracellular Ca2+ {(Ca2+)i} overload in H9c2 cells was measured by laser scanning confocal microscope (LSCM). Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram (ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression, but increased Ca2+-Mg2+-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca2+)i overload to protect cells. Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial infarct size, restoring ST segment and reversing (Ca2+)i overload. It suggests that SGI may be a new clinical candidate to treat myocardial infarction.
LanguageEnglish
Pages1097-1104
Number of pages8
JournalTropical Journal of Pharmaceutical Research
Volume16
Issue number5
DOIs
StatePublished - 2017

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Myocardial Infarction
Ions
Calcium
Injections
Calcium-Calmodulin-Dependent Protein Kinase Type 2
Ca(2+) Mg(2+)-ATPase
Calmodulin
shenxiong glucose
Serum
Intravenous Administration
Doxorubicin
Coronary Vessels
Myocardium
Electrocardiography
Lasers
Messenger RNA
Wounds and Injuries
Enzymes

Cite this

Wang, Zhi-Hua ; Pan, Jian Hao ; Ma, Xian Peng ; Xu, Xiao Yun ; Yu, Wen Hui ; Fu, Wen Juan ; Ke, Jian ; Bi, Chang Qiong ; Wei, Wei ; Zhao, Qu ; Wang, Feng-Yun ; Tang, Dan ; Ye, Kaihe ; Yi, Zhixian ; Nie, Hong. / Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload. In: Tropical Journal of Pharmaceutical Research. 2017 ; Vol. 16, No. 5. pp. 1097-1104
@article{e43dc0c0d1de4f90bb79788c696ecbfc,
title = "Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload",
abstract = "Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose Injection (SGI) in rat acute myocardial infarction (AMI). Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’ consecutive intravenous administration of SGI, serum samples were used to conduct biochemical analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology and qPCR studies. Intracellular Ca2+ {(Ca2+)i} overload in H9c2 cells was measured by laser scanning confocal microscope (LSCM). Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram (ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression, but increased Ca2+-Mg2+-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca2+)i overload to protect cells. Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial infarct size, restoring ST segment and reversing (Ca2+)i overload. It suggests that SGI may be a new clinical candidate to treat myocardial infarction.",
keywords = "Calmodulin, Calmodulin-dependent protein kinase II, Intracellular Ca overload, Ligustrazine, Myocardial infarction, Shenxiong glucose injection, Tanshinol",
author = "Zhi-Hua Wang and Pan, {Jian Hao} and Ma, {Xian Peng} and Xu, {Xiao Yun} and Yu, {Wen Hui} and Fu, {Wen Juan} and Jian Ke and Bi, {Chang Qiong} and Wei Wei and Qu Zhao and Feng-Yun Wang and Dan Tang and Kaihe Ye and Zhixian Yi and Hong Nie",
year = "2017",
doi = "10.4314/tjpr.v16i5.18",
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Wang, Z-H, Pan, JH, Ma, XP, Xu, XY, Yu, WH, Fu, WJ, Ke, J, Bi, CQ, Wei, W, Zhao, Q, Wang, F-Y, Tang, D, Ye, K, Yi, Z & Nie, H 2017, 'Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload' Tropical Journal of Pharmaceutical Research, vol. 16, no. 5, pp. 1097-1104. DOI: 10.4314/tjpr.v16i5.18

Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload. / Wang, Zhi-Hua; Pan, Jian Hao; Ma, Xian Peng; Xu, Xiao Yun; Yu, Wen Hui; Fu, Wen Juan; Ke, Jian; Bi, Chang Qiong; Wei, Wei; Zhao, Qu; Wang, Feng-Yun; Tang, Dan; Ye, Kaihe; Yi, Zhixian; Nie, Hong.

In: Tropical Journal of Pharmaceutical Research, Vol. 16, No. 5, 2017, p. 1097-1104.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cardioprotective effect of shenxiong glucose injection on acute myocardial infarction in rats via reduction in myocardial intracellular calcium ion overload

AU - Wang,Zhi-Hua

AU - Pan,Jian Hao

AU - Ma,Xian Peng

AU - Xu,Xiao Yun

AU - Yu,Wen Hui

AU - Fu,Wen Juan

AU - Ke,Jian

AU - Bi,Chang Qiong

AU - Wei,Wei

AU - Zhao,Qu

AU - Wang,Feng-Yun

AU - Tang,Dan

AU - Ye,Kaihe

AU - Yi,Zhixian

AU - Nie,Hong

PY - 2017

Y1 - 2017

N2 - Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose Injection (SGI) in rat acute myocardial infarction (AMI). Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’ consecutive intravenous administration of SGI, serum samples were used to conduct biochemical analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology and qPCR studies. Intracellular Ca2+ {(Ca2+)i} overload in H9c2 cells was measured by laser scanning confocal microscope (LSCM). Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram (ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression, but increased Ca2+-Mg2+-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca2+)i overload to protect cells. Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial infarct size, restoring ST segment and reversing (Ca2+)i overload. It suggests that SGI may be a new clinical candidate to treat myocardial infarction.

AB - Purpose: To explore the cardioprotective effects and potential mechanisms of Shenxiong Glucose Injection (SGI) in rat acute myocardial infarction (AMI). Methods: AMI model was created by ligating left anterior descending coronary artery. After 7 days’ consecutive intravenous administration of SGI, serum samples were used to conduct biochemical analysis while hearts were excised and processed for infraction size, enzyme activity, histopathology and qPCR studies. Intracellular Ca2+ {(Ca2+)i} overload in H9c2 cells was measured by laser scanning confocal microscope (LSCM). Results: In AMI rats, pretreatment with SGI significantly ameliorated myocardial histopathologic damage. It exerted cardioprotective effect by decreasing myocardial infarct size, electrocardiogram (ECG) ST segment elevation, and CK, cTnI, BNP levels in serum. In addition, SGI significantly decreased calmodulin (CaM) and calmodulin-dependent protein kinase II (CaMK II) mRNA expression, but increased Ca2+-Mg2+-ATPase and Na+-K+-ATPase activities in myocardium. In doxorubicin (DOX)-induced H9c2 cells injury model, SGI reversed (Ca2+)i overload to protect cells. Conclusion: The results demonstrate SGI exerts cardioprotective effect by decreasing myocardial infarct size, restoring ST segment and reversing (Ca2+)i overload. It suggests that SGI may be a new clinical candidate to treat myocardial infarction.

KW - Calmodulin

KW - Calmodulin-dependent protein kinase II

KW - Intracellular Ca overload

KW - Ligustrazine

KW - Myocardial infarction

KW - Shenxiong glucose injection

KW - Tanshinol

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